RT Journal Article ID 4686ae1222991c0f A1 French, Christine A1 LC, Chandra A1 Clarke, Stephen L . A1 Lucas, Edralin A. A1 Smith, Brenda J. A1 Lightfoot, Stanley A1 Kuvibidila, Solo R. T1 The Inhibitory Properties of Ethanol Extracts of Some Culinary-Medicinal Mushrooms on the Secretion of Interleukin-8 and Vascular Endothelial Growth Factor by PC3 Cancer Cells JF International Journal of Medicinal Mushrooms JO IJM YR 2019 FD 2019-07-17 VO 21 IS 7 SP 645 OP 656 K1 Agaricus bisporus K1 angiogenesis K1 interleukin-8 (IL-8) K1 Lentinus edodes K1 medicinal mushrooms K1 PC3 cells K1 prostate cancer K1 vascular endothelial growth factor (VEGF) AB Interleukin (IL)-8, a cytokine produced by immune and non-immune cells, induces angiogenesis via increased vascular endothelial growth factor (VEGF) secretion; both cytokines promote tumor growth. IL-8 and VEGF plasma levels correlate with prostate cancer severity, suggesting that therapeutic options aimed at their downregulation may modulate tumor growth. Available data suggest that Agaricus bisporus (white button mushroom [WBM]) extracts inhibit cancer cell proliferation through aromatase inhibition. However, the extent to which they affect IL-8 and VEGF remains to be elucidated. The aims of this study were to (1) investigate the antiproliferative properties of WBM, brown A. bisporus (portabella), and Lentinus edodes (shiitake mushroom) on PC3 cancer cells; (2) demonstrate that these properties are exerted through the regulation of both IL-8 and VEGF; and (3) determine the role of NFκB activation in the antiproliferative process of mushroom extracts. Cytokine secretion in the supernatant, NFκB activity, and cell proliferation were measured in PC3 cells incubated with 0−100 µg/mL of ethanol extracts of mushrooms. Mushroom extracts decreased IL-8 secretion and cell proliferation (P < .05), and also tended to decrease VEGF (P < .09). Decreased cell proliferation did not appear to result from cell death because trypan blue exclusion tests showed comparable cell viability among cultures. Mushroom extracts also decreased nuclear and total NFκB activity, and the ratio of nuclear to cytoplasmic activity (P < .05) suggesting altered translocation from the cytoplasm to the nucleus. Our data suggest that the three types of studied mushrooms may modulate tumor growth through inhibition of IL-8, VEGF, and NFκB pathways. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,4889d09f58e41d3c,4686ae1222991c0f.html