RT Journal Article
ID 46d5c1db7e0fb06f
A1 Zolj, Sanda 
A1 Smith, Melissa P. 
A1 Goines, Jillian C. 
A1 Ali, T'Shura S. 
A1 Huff, Mary O. 
A1 Robinson, David L.
A1 Lau, Joann M. 
T1 Antiproliferative Effects of a Triterpene-Enriched Extract from Lingzhi or Reishi Medicinal Mushroom, <i>Ganoderma lucidum</i> (Agaricomycetes), on Human Lung Cancer Cells
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-11-30
VO 20
IS 12
SP 1173
OP 1183
K1 apoptosis
K1 cell cycle proteins
K1 <i>Ganoderma lucidum</i>
K1 lung cancer
K1 medicinal mushrooms
K1 triterpenoids
AB <i>Ganoderma lucidum</i>, a mushroom that has been used to treat disease in East Asia for centuries, has been shown to be effective against many types of tumors, but the exact cellular mechanism of action is unknown. In this study we examined proliferation of a lung cancer cell line after treatment with 12 concentrations of powdered
<i>G. lucidum</i> for 24, 48, and 120 hours. Based on half-maximal inhibitory concentrations values, proliferation of the H1793 cell line seemed to be sensitive to the extract in a time- and dose-dependent manner. We used immunoblot analysis to examine the amounts of cell cycle proteins (cyclin D, Cdk4, and Cdc2) and apoptotic proteins (Bcl-xL and Bax) after treatment with a range of <i>G. lucidum </i>concentrations. Changes in amounts of proteins that regulate the cell cycle were consistent with longer G<sub>1</sub> and G<sub>2</sub> phases. Proapoptotic protein (Bax) levels increased 6.5-fold, with a commensurate
increase in the Bax-to-Bcl ratio, especially at 48 and 120 hours. These results suggest that the decrease in cellular proliferation correlated with a change in both cell cycle progression and apoptosis, and that the triterpenoid in <i>G. lucidum</i> is the bioactive component. Further biochemical characterization of this ancient herbal remedy could hold promise for treating lung cancer.

PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2c6ab3993645ca9e,46d5c1db7e0fb06f.html