RT Journal Article
ID 71c395cb7fecb5b9
A1 Hu, Shu-Hui
A1 Lien, Juang-Lin
A1 Hsieh, Shu-Ling
A1 Wang, Jinn-Chyi
A1 Chang, Sue-Joan
T1 Antioxidant and Antigenotoxicity Activities of Extracts from Liquid Submerged Culture of Culinary-Medicinal Ferula Oyster Mushroom, Pleurotus eryngii (DC.) Quél. var. ferulae (Lanzi) Sacc. (Agaricomycetideae)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2009
FD 2009-11-16
VO 11
IS 4
SP 395
OP 408
K1 medicinal mushrooms
K1 Pleurotus eryngii var. ferulae
K1 antigenotoxicity
K1 antimutagenicity
K1 antioxidant
K1 Ames test
K1 rec-assay
AB The ferula oyster mushroom (Pleurotus eryngii var. ferulae) is medicinal, edible, and delicious. Response surface methodology was used to determine the optimal conditions for the production of P. eryngii var. ferulae biomass in liquid submerged culture. Lyophilized mycelium was subjected to two water extracts and two elutes through column chromatography using methanol-ethyl acetate-dichloromethane (referred to as EAM and CHE, respectively). In addition, the crude protein and water-soluble polysaccharides were extracted from the cultured broth. Six samples were tested to explore their antigenotoxicity with the Ames test and a rec-assay, as well as antioxidant activities in in vivo and in vitro tests. EAM had the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging activity and ferric-reducing ability. Moreover, hamster serum showed the highest activities of glutathione peroxidase and superoxide dismutase after EAM was administered at a dose of 0.3 g kg−1 body weight daily among all tested samples. Meanwhile, the EAM group displayed significantly higher activities of the two enzymes than did the control group. In the antimutagenicity test, EAM exhibited the highest inhibition rate against every tested mutagen at a low concentration of 0.08 mg mL−1 and a high concentration of 0.3 mg mL−1 in each plate, and it also had the highest anti-DNA-damaging activity among all test samples. EAM displayed the highest antigenotoxicity effect, followed by CHE, when using water-insoluble extracts, and CP was the highest one, followed by water extract (100°C, 30 minutes), water extract (60°C, 30 minutes), and SPPF, when using water-soluble extracts. The major constituents of EAM and CHE were identified as ergosterol and 2-pentanol, respectively. Some extracts from P. eryngii var. ferulae have significant antioxidant activity and are strong antigenotoxic agents.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,4554be083929b23c,71c395cb7fecb5b9.html