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Journal of Environmental Pathology, Toxicology and Oncology
Импакт фактор: 1.241 5-летний Импакт фактор: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Печать: 0731-8898
ISSN Онлайн: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v21.i3.40
10 pages

Antioxidant Properties of Apple Juice and Its Protection Against Cr( VI)-Induced Cellular Injury

David Shi
Morgantown High School, 109 Wilson Avenue, Morgantown, WV 26505
Bing-Hua Jiang
Mary Babb Randolph Cancer Center, Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506, USA

Краткое описание

Reactive oxygen species (ROS) have been shown to cause a broad spectrum of damage to the biological system. ROS-mediated reactions are believed to play a key role in Cr(VI)-induced carcinogenesis. Using electron spin resonance (ESR) spin trapping, the present study shows that apple juice efficiently scavenged hydroxyl radicals generated by Cr(VI) reduction catalyzed by glutathione reduc-tase/NADPH. Apple juice reduced Cr(VI)-induced lipid peroxidation, DNA damage, cell apoptosis, and NF-kВ activation in human lung epithelial A549 cells. The lipid peroxidation was measured by a colorimetric assay, DNA damage by single cell gel electrophoresis assay, induction of cell apoptosis by flow cytometry, and activation of NF-kВ by luciferase assay. The results show that through its antioxidant properties, apple juice can protect Cr(VI)-induced cellular injury and may help reduce its carcinogenic potential.


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