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Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Journal of Environmental Pathology, Toxicology and Oncology
Импакт фактор: 1.241 5-летний Импакт фактор: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Печать: 0731-8898
ISSN Онлайн: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2019026470
pages 253-270

Oxidative Stress Parameters, Selenium Levels, DNA Damage, and Phthalate Levels in Plastic Workers

Gulru Gurdemir
Department of Toxicology, Faculty of Pharmacy, Hacettepe University Ankara, Turkey; Turkish Medicines and Medical Devices Agency, Ankara, Turkey
Pinar Erkekoglu
Department of Toxicology, Faculty of Pharmacy, Hacettepe University Ankara, Turkey
Aylin Balci
Department of Toxicology, Faculty of Pharmacy, Hacettepe University Ankara, Turkey
Unzile Sur
Department of Toxicology, Faculty of Pharmacy, Hacettepe University Ankara, Turkey; Department of Toxicology, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey
Gizem Ozkemahli
Department of Toxicology, Faculty of Pharmacy, Hacettepe University Ankara, Turkey; Department of Toxicology, Faculty of Pharmacy, Erzincan University, Erzincan, Turkey
Engin Tutkun
Department of Public Health, Bozok University, Yozgat, Turkey
Hinc Yilmaz
Department of Public Health, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey
Ali Asci
Department of Toxicology, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey
Belma Kocer-Gumusel
Department of Toxicology, Faculty of Pharmacy, Lokman Hekim University Ankara, Turkey

Краткое описание

Di(2-ethylhexyl)phthalate (DEHP) is the most widely used phthalate. DEHP is highly used in PVC floorings and PVC windows and carpeting. The objective of this study was to determine sex hormone levels, oxidative stress parameters, selenium levels, DNA damage, and phthalate levels in plastics workers (n = 24, age = 20-58 years) working in the production of rubber mechanical goods and exposed to DEHP in workplace. The control group (n = 29, age = 25-54, all male) was selected from age-matched healthy adults. Antioxidant parameters and DNA damage were determined by spectrophotometry. Selenium levels were determined by atomic absorption spectroscopy. Plasma hormone levels were measured by chemiluminescence microparticle immunoassay. Plasma phthalate levels were determined by high-pressure liquid chromatography. Plastic workers had lower serum testosterone and free T4 levels and higher follicle-stimulating hormone levels vs. controls. Liver enzyme activities were markedly higher in workers vs. controls. There were also increases in plasma glutathione peroxidase levels and marked decreases in plasma selenium and erythrocyte total glutathione levels in plastics workers (P < 0.05 vs. control). Plasma 8-hydroxy-2'-deoxyguanosine levels were 14-fold higher in plastics workers than in controls. Plasma DEHP and mono(2-ethylhexyl)phthalate were also markedly higher in workers vs. controls. The results of this study show that occupational exposure to DEHP may lead to disturbances in sex hormones, increased liver problems, higher oxidative stress and DNA damage levels, and lower trace element concentrations in workers. More comprehensive and mechanistic studies with higher numbers of subjects are needed to show the unwanted effects of occupational exposure to DEHP.

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