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Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Journal of Environmental Pathology, Toxicology and Oncology
Импакт фактор: 1.625 5-летний Импакт фактор: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Печать: 0731-8898
ISSN Онлайн: 2162-6537

Выпуски:
Том 39, 2020 Том 38, 2019 Том 37, 2018 Том 36, 2017 Том 35, 2016 Том 34, 2015 Том 33, 2014 Том 32, 2013 Том 31, 2012 Том 30, 2011 Том 29, 2010 Том 28, 2009 Том 27, 2008 Том 26, 2007 Том 25, 2006 Том 24, 2005 Том 23, 2004 Том 22, 2003 Том 21, 2002 Том 20, 2001

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v28.i2.40
pages 121-131

Dietary Curcumin Enhances Benzo(a)pyrene-Induced Apoptosis Resulting in a Decrease in BPDE-DNA Adducts in Mice

Rachana Garg
Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai-410 208, India
Girish Maru
Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai-410210, India

Краткое описание

Curcumin pretreatment has been shown to decrease the formation of B(a)P-derived DNA adducts; however, its effects on disappearance of BPDE-DNA adducts in vivo remain unexplored. We investigate the effect of curcumin on persistence of BPDE-DNA adducts and cell turnover in mouse tissues. Mice administered 1 mg B(a)P (gavage) were randomized after 24 h into group A (sacrificed at zero time), group B (continued on control diet), and group C (shifted to 0.05% curcumin diet), and sacrificed after 24, 48, and 96 h. Compared to group A, a time-dependent decrease in DNA adducts was observed in liver/lungs of group B mice. Mice shifted to curcumin diet showed a relatively higher decrease in DNA adducts when compared to group A/time-matched controls (group B). To investigate if this enhanced decrease was due to dilution by newly synthesized DNA or due to cell turnover; rate of DNA synthesis and apoptosis were evaluated. Similar levels of 3H-thymidine incorporated in tissue DNA from group B/C ruled out the possibility of adduct dilution. Comparative evaluation of apoptosis-related parameters showed increased apoptotic-index, p53, Bax and Caspase-3 protein expression and decreased Bcl2 levels in group C than in group A/B. This implies that curcumin post-treatment augments apoptosis of adducted cells resulting in enhanced decrease in DNA adducts.

Ключевые слова: apoptosis, benzo(a)pyrene, curcumin, DNA adducts

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