Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Journal of Environmental Pathology, Toxicology and Oncology
Импакт фактор: 1.241 5-летний Импакт фактор: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Печать: 0731-8898
ISSN Онлайн: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2015013074
pages 191-200

Generation of Reactive Oxygen Species and Endoplasmic Reticulum Stress by Dictyopteris undulata Extract Leads to Apoptosis in Human Melanoma Cells

Joon Ki Kim
Department of Bio and Nanochemistry, Kookmin University, Seoul, Korea
Kyoung Ah Kang
School of Medicine, Jeju National University, Jeju, Republic of Korea
Mei Jing Piao
School of Medicine, Jeju National University, Jeju, Republic of Korea
Madduma Hewage Susara Ruwan Kumara
School of Medicine, Jeju National University, Jeju, Korea
Yong Joo Jeong
Department of Bio and Nanochemistry, Kookmin University, Seoul, Korea
Mi Hee Ko
Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea
Jin Won Hyuna
School of Medicine, Jeju National University, Jeju, Republic of Korea

Краткое описание

In this study, we evaluated the hypothesis that a marine brown algae, Dictyopteris undulata ethanol extract (DUE), provokes apoptosis in a human melanoma cell line, A2058, via reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress. DUE inhibited A2058 cell proliferation and increased apoptotic body formation, as indicated by the presence of fragmented nuclei and the activation of caspase-3. Moreover, DUE-treated cells showed elevated ER staining, mitochondrial calcium cation (Ca2+) overloading, augmented levels of ER stress-related and cell death modulatory proteins, including RNA-dependent protein kinase-related ER kinase, phospho-inositol-requiring enzyme 1α, phospho-eukaryotic translation initiation factor 2α, and CCAAT/enhancer-binding protein-homologous protein, as well as increased intracellular ROS levels. However, the antioxidant N-acetyl cysteine reversed the elevated ROS levels, decreased apoptosis, and mitigated ER stress in A2058 cells following DUE treatment. These findings suggest that DUE treatment triggers apoptosis in human melanoma cells through a mechanism involving ER stress and ROS.


Articles with similar content:

Redox Status-Dependent Regulation of Cyclooxygenases Mediates the Capsaicin-Induced Apoptosis in Human Neuroblastoma Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.21, 2002, issue 2
Young Shin Kang, Seung Hee Park, Keun Huh, Da Qing Jin, Eun Jin Kwon, Yong Soo Lee, Jung-Ae Kim
Nuclear Factor Kappa B: A Pro-Inflammatory, Transcription Factor−Mediated Signalling Pathway in Lung Carcinogenesis and Its Inhibition By Nonsteroidal Anti-Inflammatory Drugs
Journal of Environmental Pathology, Toxicology and Oncology, Vol.31, 2012, issue 1
Sankar Nath Sanyal, Shruti Setia
Targeting MAPK (p38, ERK, JNK) and inflammatory CK (GDF-15, GM-CSF) in UVB-Activated Human Skin Cells with Vitis vinifera Seed Extract
Journal of Environmental Pathology, Toxicology and Oncology, Vol.37, 2018, issue 3
Corina Bianca Tatomir, Maria Perde-Schrepler, Piroska Virag, Ioana Carmen Brie, Hana Petra Decean, Eva Fischer-Fodor
Protective Role of Curcumin and Flunixin Against Acetic Acid−Induced Inflammatory Bowel Disease via Modulating Inflammatory Mediators and Cytokine Profile in Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.34, 2015, issue 4
G BOOBALAN, Matukumalli Usha Rani, C.S.V. Satish Kumar, Alla Gopala Reddy, Rasakatla Dileep, Matham Vijay Kumar
The Role of Nitric Oxide and Taurine in Regulation of Gastric Secretory Function in Dogs
International Journal of Physiology and Pathophysiology, Vol.4, 2013, issue 3
Olga A. Grinchenko , Petro I. Yanchuk