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ISSN Печать: 0731-8898
ISSN Онлайн: 2162-6537
Indexed in
Bacillus thuringiensis Parasporal Proteins Induce Cell-Cycle Arrest and Caspase-Dependant Apoptotic Cell Death in Leukemic Cells
Краткое описание
Bacillus thuringiensis (Bt) parasporal proteins with selective anticancer activity have recently garnered interest. This study determines the efficacy and mode of cell death of Bt 18 parasporal proteins against 3 leukemic cell lines (CEM-SS, CCRF-SB and CCRF-HSB-2).Cell-based biochemical analysis aimed to determine cell viability and the percentage of apoptotic cell death in treated cell lines; ultrastructural analysis to study apoptotic changes and Western blot to identify the parasporal proteins' binding site were performed. Bt 18 parasporal proteins moderately decreased viability of leukemic cells but not that of normal human T lymphocytes. Further purification of the proteins showed changes in inhibition selectivity. Phosphatidylserine externalization, active caspase-3, cell cycle, and ultrastructural analysis confirmed apoptotic activity and S-phase cell-cycle arrest. Western blot analysis demonstrated glyceraldehyde 3-phosphate dehydrogenase as a binding protein. We suggest that Bt 18 parasporal proteins inhibit leukemic cell viability by cell-cycle arrest and apoptosis and that glyceraldehyde 3-phosphate dehydrogenase binding initiates apoptosis.
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Franco Molina M A, Mendoza Gamboa E, Roman Calderon M E, Zapata Benavides P, Rivera Morales L G, Zapata Monsivais L, Coronado ndash Cerda E E, Sierra Rivera C A, Tamez Guerra R, Rodr iacute guez Padilla C, In vitro antitumoral activity of soluble protein extracts of Bacillus thuringiensis, African Journal of Microbiology Research, 10, 10, 2016. Crossref
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Gurnev Philip, Nestorovich Ekaterina, Channel-Forming Bacterial Toxins in Biosensing and Macromolecule Delivery, Toxins, 6, 8, 2014. Crossref
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Chi Xiaoyuan, Su Peng, Bi Dan, Tai Zhao, Li Yingying, Pang Yue, Li Qingwei, Lamprey immune protein-1 (LIP-1) from Lampetra japonica induces cell cycle arrest and cell death in HeLa cells, Fish & Shellfish Immunology, 75, 2018. Crossref
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Beena V., Ramnath V., Sreekumar K. P., Karthiayini K., Philomina P. T., Girija D., Crystal Protein of a Novel Bacillus thuringiensis Strain Inducing Cell Cycle Arrest and Apoptotic Cell Death in Human Leukemic Cells, Scientific Reports, 9, 1, 2019. Crossref
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Chu Wan-Loy, Tan Eng-Lai, Ambu Stephen, Leong Chee-Onn, Nadarajah Vishna Devi, Lim Patricia Kim-Chooi, Wong Shew-Fung, Khor Geok-Lin, Menke James Michael, Mak Joon-Wah, Institute for Research, Development and Innovation (IRDI) of the International Medical University (IMU), Malaysia, in The Malaysia-Japan Model on Technology Partnership, 2015. Crossref
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Hernandez-Salas Yajaira, Hernandez-Teran Fernando, Balaguramy Nagamani, De La Fuente-Salcido Norma, Alternativa biotecnológica contra el cáncer: Parasporinas de Bacillus thuringiensis, Revista de Ingeniería Biomédica y Biotecnología, 2019. Crossref