Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Journal of Environmental Pathology, Toxicology and Oncology
Импакт фактор: 1.241 5-летний Импакт фактор: 1.349 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN Печать: 0731-8898
ISSN Онлайн: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v29.i2.50
pages 113-125

Diallyl Disulfide Induces Caspase-Dependent Apoptosis via Mitochondria-Mediated Intrinsic Pathway in B16F-10 Melanoma Cells by Up-Regulating P53, Caspase-3 and Down-Regulating Pro-Inflammatory Cytokines and Nuclear Factor- κβ-Mediated Bcl-2 Activation

P. Pratheeshkumar
Amala Cancer Research Centre, Amala Nagar, Thrissur-680555, Kerala State, India
P. Thejass
Amala Cancer Research Centre, Amala Nagar, Thrissur-680555, Kerala State, India
Girija Kuttan
Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala State, India

Краткое описание

Diallyl disulfide (DADS) is a major organo-sulfur compound derived from garlic (Allium sativum), which inhibits the proliferation of various types of cancer cells. In this study we investigated the effect of DADS on the induction of apoptosis, as well as its regulatory effect on the activation of transcription factors in B16F-10 melanoma cells. Treatment of B16F-10 cells with nontoxic concentrations of DADS resulted in the presence of apoptotic bodies and induced DNA fragmentation in a dose-dependent manner. Cell-cycle analysis revealed that the occurrence of the sub-G1 peak was significantly elevated in DADS-treated cells. DADS treatment also down-reguated Bcl-2 expression and up-regulated p53, caspase-9, and caspase-3 expression in B16F-10 melanoma cells. The study also reveals that DADS inhibited the activation and nuclear translocation of p65, p50, and c-Rel subunits of nuclear factor (NF)-ℵB and other transcription factors, such as c-fos, activated transcription factor-2, and cyclic adenosine monophosphate response element-binding protein, in B16F-10 melanoma cells The pro-inflammatory cytokine production and gene expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and granulocyte-macrophage colony-stimulating factor (GM-CSF) were down-regulated in DADS-treated cells compared with control B16F-10 metastatic melanoma cells. DADS induces caspase-dependent apoptosis through a mitochondria-mediated intrinsic pathway in B16F-10 melanoma cells by activating p53 and caspase-3 gene expression and suppressing pro-inflammatory cytokines and NF-ℵB-mediated Bcl-2 activation.

Articles with similar content:

13-cis-Retinoic Acid Induces Apoptosis by Modulating Caspase-3, bcl-2, and p53 Gene Expression and Regulates the Activation of Transcription Factors in B16F-10 Melanoma Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.27, 2008, issue 3
Chaluvally Raghavan Pradeep, Chandrasekharan Guruvayoorappan, Girija Kuttan
Protective Effect of Solanum muricatum on Tumor Metastasis by Regulating Inflammatory Mediators and Nuclear Factor-Kappa B Subunits
Journal of Environmental Pathology, Toxicology and Oncology, Vol.34, 2015, issue 3
Chandrasekaran Guruvayoorappan, Kittappa Shathish, Kunnathur Murugesan Sakthivel
A Polysaccharide from the Culinary-Medicinal Mushroom Pholiota nameko (Agaricomycetes) Inhibits the NF-κB Pathway in Dendritic Cells Through the TLR2 Receptor
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 11
Guoliang Li, Lihua Huai, Yufeng Xu, Haiping Li, Ruixuan Zhu, Fengling Wang, Pei Zhao
The Agaricoglyceride of Royal Sun Medicinal Mushroom, Agaricus brasiliensis (Higher Basidiomycetes) is Anti-Inflammatory and Reverses Diabetic Glycemia in the Liver of Mice
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 4
Xu Gao, Yan Sun, Haitao Yu, Xiaodan Qi, Chunchao Han, Chunjing Zhang, Yan Shi
Ipobscurine, an Indole Alkaloid from Ipomoea obscura, Inhibits Tumor Cell Invasion and Experimental Metastasis by Inducing Apoptosis
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 2
T. P. Hamsa, Girija Kuttan