Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Critical Reviews™ in Oncogenesis
SJR: 0.946 SNIP: 0.503 CiteScore™: 2

ISSN Печать: 0893-9675
ISSN Онлайн: 2162-6448

Critical Reviews™ in Oncogenesis

DOI: 10.1615/CritRevOncog.v5.i4.10
pages 331-357

SV40-lnduced Immortalization of Human Cells

Tracy Bryan
Children's Medical Research Institute
Roger R. Redder
Children's Medical Research Institute, 214 Hawkesbury Road, Westmead, Sydney, NSW 2145, Australia

Краткое описание

For several decades simian virus 40 (SV40) early region genes have been used as a means of generating immortalized human cell lines; however, the molecular mechanisms of this process have begun to be understood only recently. SV40-induced immortalization proceeds via two phases. In the first phase ("lifespan extension"), cells continue proliferating for a limited number of population doublings beyond the point at which normal cells undergo senescence. This is mainly due to the ability of SV40 large T antigen (LTAg) to bind to the protein products of the p53 and retinoblastoma (Rb) genes. The second phase ("immortalization") occurs in only a small minority of cells, and cell hybridization analyses indicate that this is a gene inactivation event. The gene or genes involved are currently unknown, but chromosomal localization data are accumulating which should make their cloning and characterization possible in the near future.


Articles with similar content:

Regulation of the Chicken Lysozyme Locus in Transgenic Mice
Critical Reviews™ in Eukaryotic Gene Expression, Vol.6, 1996, issue 2-3
Nicole Faust, Matthias C. Huber, Albrecht E. Sippel, Constanze Bonifer
A Compilation and Classification of DNA Binding Sites for Protein Transcription Factors from Vertebrates
Critical Reviews™ in Eukaryotic Gene Expression, Vol.4, 1994, issue 2-3
Teni Boulikas
Expression of the BMP 2 Gene during Bone Cell Differentiation
Critical Reviews™ in Eukaryotic Gene Expression, Vol.4, 1994, issue 2-3
Stephen E. Harris, Gregory R. Mundy, Marie A. Harris, Nandini Ghosh-Choudhury, Jian Q. Feng
Cyclins, Cyclin-Dependent Kinases and Cdk Inhibitors: Implications in Cell Cycle Control and Cancer
Critical Reviews™ in Eukaryotic Gene Expression, Vol.5, 1995, issue 2
Antonio Giordano, Timothy K. MacLachlan, Nianli Sang
Molecular Biology of Muscarinic Acetylcholine Receptors
Critical Reviews™ in Neurobiology, Vol.10, 1996, issue 1
Jurgen Wess