Выходит 6 номеров в год
ISSN Печать: 0278-940X
ISSN Онлайн: 1943-619X
Indexed in
Generation of Acsl4 Gene Knockout Mouse Model by CRISPR/Cas9-Mediated Genome Engineering
Get access
Краткое описание
Acyl-CoA synthetase 4 (Acsl4) is involved in lipid synthesis and fatty acid degradation, and disruption of its function causes lipid metabolism disorder in various species. Herein, we report the generation of Acsl4 knockout (KO) mice using the CRISPR/Cas9 gene editing system to study its effects on lipid deposition. In this report, a large 12kb deletion in the Acsl4 gene was performed by coinjection of Cas9 mRNA and two guide RNAs (sgRNAs) into mouse fertilized oocytes. Six mutant mice carrying target mutations were examined by PCR analysis and direct sequencing. The gene modified mice remained healthy and displayed normal behavior. All the mutant F0 mice were mated with wild mice to produce the F1 generation, and only 1 F1 mutant mouse was obtained.
-
F e r n a n d e z X , M o n i n G , T a l m a n t A , M o u r o t J , L e b r e t B . I n f l u e n c e o f i n t r a m u s c u l a r f a t c o n t e n t o n t h e q u a l i t y o f p i g m e a t - 2 . C o n s u m e r a c c e p t a b i l i t y o f m . l o n g i s s i m u s l u m b o r u m . M e a t S c i . 1 9 9 9 ; 5 3 ( 1 ) : 6 7 - 7 2 . .
-
M e r c a d e A , P e r e z - E n c i s o M , V a r o n L , A l v e s E , N o g u e r a J L , S a n c h e z A S , F o l c h J M . A d i p o c y t e f a t t y - a c i d b i n d i n g p r o t e i n i s c l o s e l y a s s o c i a t e d t o t h e p o r c i n e F A T 1 l o c u s o n c h r o m o s o m e 4 . J A n i m S c i . 2 0 0 6 ; 8 4 ( 1 1 ) : 2 9 0 7 - 1 3 . .
-
M e r c a d e A , S a n c h e z A , F o l c h J M . A s s i g n m e n t o f t h e a c y l - C o A s y n t h e t a s e l o n g - c h a i n f a m i l y m e m b e r 4 ( A C S L 4 ) g e n e t o p o r c i n e c h r o m o s o m e X . A n i m G e n e t . 2 0 0 5 ; 3 6 ( 1 ) : 7 6 . .
-
C h e n J N , J i a n g Y Z , C e n W M , X i n g S H , Z h u L , T a n g G Q , L i M Z , J i a n g A A , L o u P E , W e n A X , W a n g Q , H e T , Z h u G X , X i e M , L i X W . D i s t r i b u t i o n o f H - F A B P a n d A C S L 4 g e n e p o l y m o r p h i s m s a n d t h e i r a s s o c i a t i o n s w i t h i n t r a m u s - c u l a r f a t c o n t e n t a n d b a c k f a t t h i c k n e s s i n d i f f e r e n t p i g p o p u l a t i o n s . G e n e t M o l R e s . 2 0 1 4 ; 1 3 ( 3 ) : 6 7 5 9 - 7 2 . .
-
C h a n g N , S u n C , G a o L , Z h u D , X u X , Z h u J , X i o n g W , X i J J . G e n o m e e d i t i n g w i t h R N A - g u i d e d C a s 9 n u c l e a s e i n z e b r a f i s h e m b r y o s . C e l l R e s . 2 0 1 3 ; 2 3 ( 4 ) : 4 6 5 - 7 2 . .
-
G r a t z S J , C u m m i n g s A M , N g u y e n J N , H a m m D C , D o n o h u e L K , H a r r i s o n M M , W i l d o n g e r J , O ' C o n n o r - G i l e s K M . G e n o m e e n g i n e e r i n g o f D r o s o p h i l a w i t h t h e C R I S P R R N A - g u i d e d C a s 9 n u c l e a s e . G e n e t i c s . 2 0 1 3 ; 1 9 4 ( 4 ) : 1 0 2 9 - 3 5 . .
-
W a n g H , Y a n g H , S h i v a l i l a C S , D a w l a t y M M , C h e n g A W , Z h a n g F , J a e n i s c h R . O n e - s t e p g e n e r a t i o n o f m i c e c a r r y i n g m u t a t i o n s i n m u l t i p l e g e n e s b y C R I S P R / C a s - m e d i a t e d g e n o m e e n g i n e e r i n g . C e l l . 2 0 1 3 ; 1 5 3 ( 4 ) : 9 1 0 - 1 8 . .
-
W a n g X , C a o C , H u a n g J , Y a o J , H a i T , Z h e n g Q , Z h a n g H , Q i n G , C h e n g J , W a n g Y , Y u a n Z , Z h o u Q , W a n g H , Z h a o J . O n e - s t e p g e n e r a t i o n o f t r i p l e g e n e - t a r g e t e d p i g s u s i n g C R I S P R / C a s 9 s y s t e m . S c i R e p . 2 0 1 6 ; 6 : 2 0 6 2 0 . .
-
S o n g Y , Y u a n L , W a n g Y , C h e n M , D e n g J , L V Q , S u i T , L i Z , L a i L . E f f i c i e n t d u a l s g R N A - d i r e c t e d l a r g e g e n e d e l e t i o n i n r a b b i t w i t h C R I S P R / C a s 9 s y s t e m . C e l l M o l L i f e S c i . 2 0 1 6 ; 7 3 ( 1 5 ) : 2 9 5 9 - 6 8 . .
-
C h e n X , X u F , Z h u C , J i J , Z h o u X , F e n g X , G u a n g S . D u a l s g R N A - d i r e c t e d g e n e k n o c k o u t u s i n g C R I S P R / C a s 9 t e c h n o l o g y i n C a e n o r h a b d i t i s e l e g a n s . S c i R e p . 2 0 1 4 ; 4 : 7 5 8 1 . .
-
R a n F , P D A , H s u J , W r i g h t V , A g a r w a l a D , S c o t t A , Z h a n g F . G e n o m e e n g i n e e r i n g u s i n g t h e C R I S P R - C a s 9 s y s t e m . N a t P r o t o c . 2 0 1 3 ; 8 ( 1 1 ) : 2 2 8 1 - 2 3 0 8 . .
-
R e n H , T a o C , L i K , B i Y , Z h e n g X . G e n e r a t i o n o f a f l o x e d a l l e l e o f t h e m o u s e m i c r o R N A - 2 0 0 c l u s t e r s . A p p l B i o c h e m B i o t e c h n o l . 2 0 1 7 ; 1 8 2 ( 3 ) : 1 2 1 8 - 1 2 2 8 . .
-
W e s t r a E R , S e m e n o v a E , D a t s e n k o K A , J a c k s o n R N , W i e d e n h e f t B , S e v e r i n o v K , B r o u n s S J . T y p e I - E C R I S - P R - c a s s y s t e m s d i s c r i m i n a t e t a r g e t f r o m n o n - t a r g e t D N A t h r o u g h b a s e p a i r i n g - i n d e p e n d e n t P A M r e c o g n i t i o n . P L o S G e n e t . 2 0 1 3 ; 9 ( 9 ) : e 1 0 0 3 7 4 2 . .
-
C h o i P S , M e y e r s o n M . T a r g e t e d g e n o m i c r e a r r a n g e m e n t s u s i n g C R I S P R / C a s t e c h n o l o g y . N a t C o m m u n . 2 0 1 4 ; 5 : 3 7 2 8 . .
-
Z h o u J J , W a n g B , S h e n L , C h e n Y , S u J , Y a n g W , Z h a n g X , T i a n , H u a n g X . D u a l s g R N A s f a c i l i t a t e C R I S P R / C a s 9 - m e d i a t e d m o u s e g e n o m e t a r g e t i n g . F E B S J . 2 0 1 4 ; 2 8 1 ( 7 ) : 1 7 1 7 - 2 5 . .
-
J a n g D E , L e e J Y , L e e J H , K o o O J , B a e H S , J u n g M H , B a e J H , H w a n g W S , C h a n g Y J , L e e Y H , L e e H W , Y e o m S C . M u l t i p l e s g R N A s w i t h o v e r l a p p i n g s e q u e n c e s e n h a n c e C R I S P R / C a s 9 - m e d i a t e d k n o c k - i n e f f i c i e n c y . E x p M o l M e d . 2 0 1 8 ; 5 0 ( 4 ) : 1 6 . .
-
B a s s e t t A R , T i b b i t C , P o n t i n g C P , L i u J L . H i g h l y e f f i c i e n t t a r g e t e d m u t a g e n e s i s o f D r o s o p h i l a w i t h t h e C R I S P R / C a s 9 s y s t e m . C e l l R e p . 2 0 1 3 ; 4 ( 1 ) : 2 2 0 - 2 8 . .
-
W a n g H , Y a n g H , S h i v a l i l a C S , D a w l a t y M , C h e n g A W , Z h a n g F , J a e n i s c h R . O n e - s t e p g e n e r a t i o n o f m i c e c a r r y i n g m u t a t i o n s i n m u l t i p l e g e n e s b y C R I S P R / C a s - m e d i a t e d g e n o m e e n g i n e e r i n g . C e l l . 2 0 1 3 ; 1 5 3 ( 4 ) : 9 1 0 - 9 1 8 . .
-
Kato Ayumi, Ito Mana, Sanaki Takao, Okuda Tomohiko, Tsuchiya Noriko, Yoshimoto Ryo, Yukioka Hideo, Acsl1 is essential for skin barrier function through the activation of linoleic acid and biosynthesis of ω-O-acylceramide in mice, Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1867, 2, 2022. Crossref
-
Jamehdor Saleh, Pajouhanfar Sara, Saba Sadaf, Uzan Georges, Teimoori Ali, Naserian Sina, Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions, Cells, 11, 6, 2022. Crossref
-
Xu Lei, Lu Xi, Xiao Peng, Liu Ran, Xia Kunlong, Wu Meizhou, Jin Meilin, Zhang Anding, Interleukin-17A Contributed to the Damage of Blood-CNS Barriers During Streptococcus suis Meningitis, Molecular Neurobiology, 59, 4, 2022. Crossref
-
Xu Lei, Zeng Yue, Gao Peiying, Lu Xi, Xia Kunlong, Zhou Liting, Zhang Chengfeng, Yi Chenyang, Zhang Anding, IL-18 Signaling Is Essential for Causing Streptococcal Toxic Shock-like Syndrome (STSLS), Life, 12, 9, 2022. Crossref