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Plasma Medicine

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ISSN Печать: 1947-5764

ISSN Онлайн: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Experimental Study of Atmospheric Pressure Argon Plasma Jet−Induced Strand Breakage in Large DNA Molecules

Том 7, Выпуск 1, 2017, pp. 65-76
DOI: 10.1615/PlasmaMed.2017019986
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In recent years, the atmospheric pressure plasma jet (APPJ) has been extensively studied for potential biological and medical application. It has been shown that a bactericidal effect is primarily due to the inactivation of the DNA repair mechanism or a DNA damage response in the microorganism. The damage is attributed to the interaction of biomolecules with reactive species produced by the plasma. In this experiment, the influence of the environmental medium on the efficacy of an argon plasma jet in the breakdown of large DNA (λ DNA) molecules was studied using characterization of the APPJ by electrical and optical methods. The DNA was suspended in four different solutions: physiological saline (PS), water, phosphate buffered saline (PBS), and Tris-EDTA (TE) and exposed to a plasma jet for three different periods of time (60, 90, and 120 s). The molecular responses of λ DNA were analyzed in gel electrophoresis. The largest amount of breakage was found to occur in water, with significant damage found at 120 s. PBS showed a linear degradation pattern. The free-radical scavenging properties of the medium may play a significant part in preventing strand breakage, and this must therefore be taken into account when exposing microorganisms and human cells during experiments. The crucial role of medium properties highlights the need for further studies of organisms in their natural surroundings.

Ключевые слова: atmospheric pressure argon plasma jet, DNA, PBS, water
ЦИТИРОВАНО В
  1. Ilik Erkan, Durmus Cagri, Akan Tamer, Adding Water Droplets Into Atmospheric Pressure Plasma Jet of Helium, IEEE Transactions on Plasma Science, 47, 11, 2019. Crossref

  2. Shakya Aavash , Pradhan Suman Prakash , Banset Niroj , Shrestha Rajendra , Joshi Pusp Raj , Gautam Roshan , Paneru Aakash , GC Ashok , Shah Arun Kumar , Adhikari Rameshwar , Subedi Deepak Prasad, Regmi Sagar, Characterization and Assessment of Cold Plasma for Cancer Treatment , Plasma Medicine, 12, 2, 2022. Crossref

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