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Plasma Medicine

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ISSN Печать: 1947-5764

ISSN Онлайн: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Optimum Power of Low-Temperature Plasma Selectivity for Human Melanoma Cell Treatment

Том 9, Выпуск 1, 2019, pp. 89-99
DOI: 10.1615/PlasmaMed.2019030124
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The number of new cases of melanoma is increasing every year. Due to its specificity of treatment, plasma medicine has been claimed to be a novel adjunctive procedure. We evaluated the effects of a direct low-temperature plasma (LTP) device for selective tumor eradication, comparing between melanoma and normal keratinocyte cell lines. Human melanoma (G-361) and normal keratinocyte (HaCaT) cell lines were subjected to LTP treatment. Different power levels and exposure durations of LTP were compared in both groups. After the treatment, a MTT assay was used to determine cell viability. The physical plasma characteristics of the three powers were also evaluated. It was found that low power (intensity 1 with frequency 10 Hz) was 0.25 watts, while medium power (intensity 5 with frequency 50 Hz) and high power (intensity 10, with frequency 100 Hz) were 0.94 and 3.0 watts, respectively. Higher powers and longer durations of LTP treatment increased the incidence of cell death in both the HaCaT and G-361 cell lines. LTP eliminated slightly more G-361 than HaCaT cells at 10 and 30 seconds of exposure. At 60 seconds or more, LTP showed a decrease of more than 50% in both the HaCaT and G-361 survival. Medium LTP power with 10-second exposure demonstrated a statistically significant difference between HaCaT and G-361 viabilities (77.1% and 66.3%, respectively; P = 0.01). LTP treatment at medium power (intensity 5 with frequency 50 Hz, equal to 0.94 watts) at 10-second duration showed a specificity for cancer eradication.

Ключевые слова: plasma medicine, plasma jet, melanoma, selectivity
ЛИТЕРАТУРА
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