Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Critical Reviews™ in Eukaryotic Gene Expression
Импакт фактор: 1.841 5-летний Импакт фактор: 1.927 SJR: 0.649 SNIP: 0.516 CiteScore™: 1.96

ISSN Печать: 1045-4403
ISSN Онлайн: 2162-6502

Выпуски:
Том 29, 2019 Том 28, 2018 Том 27, 2017 Том 26, 2016 Том 25, 2015 Том 24, 2014 Том 23, 2013 Том 22, 2012 Том 21, 2011 Том 20, 2010 Том 19, 2009 Том 18, 2008 Том 17, 2007 Том 16, 2006 Том 15, 2005 Том 14, 2004 Том 13, 2003 Том 12, 2002 Том 11, 2001 Том 10, 2000 Том 9, 1999 Том 8, 1998 Том 7, 1997 Том 6, 1996 Том 5, 1995 Том 4, 1994

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukaryotGeneExpr.2014010313
pages 205-223

Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: PART A−Radiomics

Martin Falk
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
Michael Hausmann
Kirchhoff Institute for Physics, University of Heidelberg, Heidelberg, Germany
Emilie Lukasova
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
Abin Biswas
Kirchhoff Institute for Physics, University of Heidelberg, Heidelberg, Germany; Department of Radiation Oncology, University Medical Center Mannheim, University of Heidelberg, Heidelberg, Germany
Georg Hildenbrand
Department of Radiation Oncology, University Medical Center Mannheim, Kirchhoff Institute for Physics, University of Heidelberg, Heidelberg, Germany
Marie Davidkova
Nuclear Physics Institute, Academy of Sciences of the Czech Republic, Rez, Czech Republic
Evgeny Krasavin
Joint Institute for Nuclear Research, Dubna, Moscow, Russia
Zdenek Kleibl
Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic
Iva Falkova
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
Lucie Jezkova
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic; Joint Institute for Nuclear Research, Dubna, Moscow, Russia; Institute of Chemical Technology Prague, Prague, Czech Republic
Lenka Stefancikova
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
Jan Sevcik
Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic
Michal Hofer
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
Alena Bacikova
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
Pavel Matula
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic; Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Brno, Czech Republic
Alla Boreyko
Joint Institute for Nuclear Research, Dubna, Moscow, Russia
Jana Vachelova
Nuclear Physics Institute, Academy of Sciences of the Czech Republic, Rez, Czech Republic
Anna Michaelidesova
Nuclear Physics Institute, Academy of Sciences of the Czech Republic, Rez, Czech Republic; Proton Therapy Center, Prague, Czech Republic
Stanislav Kozubek
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

Краткое описание

Recent ground-breaking developments in Omics have generated new hope for overcoming the complexity and variability of biological systems while simultaneously shedding more light on fundamental radiobiological questions that have remained unanswered for decades. In the era of Omics, our knowledge of how genes and proteins interact in the frame of complex networks to preserve genome integrity has been rapidly expanding. Nevertheless, these functional networks must be observed with strong correspondence to the cell nucleus, which is the main target of ionizing radiation. Nuclear architecture and nuclear processes, including DNA damage responses, are precisely organized in space and time. Information regarding these intricate processes cannot be achieved using high-throughput Omics approaches alone, but requires sophisticated structural probing and imaging. Based on the results obtained from studying the relationship between higher-order chromatin structure, DNA double-strand break induction and repair, and the formation of chromosomal translocations, we show the development of Omics solutions especially for radiation research (radiomics) (discussed in this article) and how confocal microscopy as well as novel approaches of molecular localization nanoscopy fill the gaps to successfully place the Omics data in the context of space and time (discussed in our other article in this issue, "Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: Part B−Structuromics"). Finally, we introduce a novel method of specific chromatin nanotargeting and speculate future perspectives, which may combine nanoprobing and structural nanoscopy to observe structure–function correlations in living cells in real time. Thus, the Omics networks obtained from function analyses may be enriched by real-time visualization of Structuromics.


Articles with similar content:

Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: Part B− Structuromics
Critical Reviews™ in Eukaryotic Gene Expression, Vol.24, 2014, issue 3
Lenka Stefancikova, Marie Davidkova, Abin Biswas, Jan Sevcik, Michal Hofer, Anna Michaelidesova, Iva Falkova, Emilie Lukasova, Michael Hausmann, Alena Bacikova, Zdenek Kleibl, Alla Boreyko, Pavel Matula, Jana Vachelova, Stanislav Kozubek, Evgeny Krasavin, Martin Falk, Lucie Jezkova, Georg Hildenbrand
Historical Perspective of Cell-Cell Fusion in Cancer Initiation and Progression
Critical Reviews™ in Oncogenesis, Vol.18, 2013, issue 1 - 2
George E. Parris
p53 Binding to Target Sites Is Dynamically Regulated Before and After Ionizing Radiation-Mediated DNA Damage
Journal of Environmental Pathology, Toxicology and Oncology, Vol.23, 2004, issue 1
Alex Almasan, Marcela Oancea, Meredith E. Crosby
Nuclear Structure, Gene Expression and Development
Critical Reviews™ in Eukaryotic Gene Expression, Vol.9, 1999, issue 3-4
Karen Brown
Application of DNA Microarray to Toxicological Research
Journal of Environmental Pathology, Toxicology and Oncology, Vol.23, 2004, issue 1
Toshi Shioda