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ISSN Печать: 2155-014X
ISSN Онлайн: 2155-0158
Activation of TRPV1 by Nitric Oxide Donors Requires Co-Application of Sulfhydryl-Containing Reagent
Краткое описание
Previous studies suggested that polymodal capsaicin-sensitive TRPV1 ion channel could be also a receptor for nitric oxide (NO). However, the evidence for this notion is based on rather indirect experiments, which questions its physiological relevance. The present paper provides an improved method for applying NO donors, such as sodium nitroprusside (SNP), in electrophysiological experiment, and simultaneous delivery of sulfhydryl-containing reagents, including L-cysteine (L-Cys) that facilitates the release of NO from a donor substance and allows inducing macroscopic TRPV1-mediated current. We have found that external application of 100 μM SNP per se onto capsaicin-sensitive neurons of the dorsal root ganglia (DRG) is incapable of activating TRPV1 ion channel. In contrast, the application of SNP simultaneously with 100 μM L-Cys induces transmembrane current with features characteristic of TRPV1-mediated one, i.e., inward at command membrane potential (Vcomm) +50 mV, outward at Vcomm-100 mV, and susceptible to blockade with capsazepine, a selective TRPV1 antagonist. At the same time, the application of L-Cys itself evokes no response in conditions tested. To summarize, our work confirms the assumption about the sensitivity of TRPV1 to NO and provides a simple method for further examining of their interaction.
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Amorim Mayara Alves, Jentsch Matias de Oliveira Janiana Raíza, Souza Oliveira Vitor Hélio, Cabrini Daniela Almeida, Otuki Michel Fleith, André Eunice, Role of nitric oxide, bradykinin B2 receptor, and TRPV1 in the airway alterations caused by simvastatin in rats, European Journal of Pharmacology, 912, 2021. Crossref