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Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Печать: 2155-014X
ISSN Онлайн: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v8.i1.40
pages 33-44

Mitochondrial Respiration and Oxidative Phosphorylation in Rat Tissues under Oral Taurine Injection

Roman D. Ostapiv
Ivan Franko National University, Lviv, Ukraine; State Scientific Controlling Institute Of Veterinary Medical Products And Feed Additives, Lviv, Ukraine
Volodymyr V. Manko
Ivan Franko National University, Lviv, Ukraine

Краткое описание

Taurine, sulphur-containing amino acid, is an essential component of mitochondrial matrix, where it maintains pH and is included in mitochondrial RNA transport. However, it is still unclear how taurine affects ATP synthesis and activity of mitochondrial respiratory chain components. We investigated the effect of a long-term oral administration of taurine on mitochondrial respiration and oxidative phosphorylation in the tissues of liver, brain, testes, and femoral muscle of rats. For this, male Wistar rats weighing 190 − 220 g were divided into three experimental groups. We injected either drinking water (control group), or solution of taurine (40 or 100 mg / kg to I and II research groups, respectively), daily for 28 days. The rate of mitochondrial respiration was determined by a polarographic method using the Clark electrode during the oxidation of endogenous substrates (V1), addition of exogenous α-ketoglutarate (5 mmol / l) or succinate (1 mg / l), and when ADP was added to a final concentration of 200 μmol / l (V3), and after using ADP by the mitochondria (V4ATP). It turned out that the prolonged administration of taurine resulted in V1 increase in animals of both experimental groups in the liver and the brain by 50 − 60%, but in the testes and muscles it decreased in I research group by 48 − 73%. In the liver of animals of I experimental group, after oxidation of α-ketoglutarate and succinate, the values of V4S, V3, and V4ATP were 4 − 7 times higher than the control values. In the liver of animals of II experimental group, those indices under oxidation of α-ketoglutarate were higher by 57 − 126%. In the muscles of I experimental group, α-ketoglutarate and succinate evoked lowering of V3 and V4ATP by 41.4 − 60.9%, while in the muscles of animals of II experimental group, oxidation of α-ketoglutarate V3 was higher by 23.7%. Adding of succinate evoked V4S and V4ATP increase by 31 − 70% in the testes of animals of both experimental groups and in the brain of I experimental group. However, in the brains of II experimental group, respiration intensity (V4S), was lower by 38.3%. Thus, the effect of prolonged administration of taurine on the intensity of oxygen consumption by the mitochondria is dose-dependent and tissue-specific. The latter obviously is of different significance and is implemented by different mechanisms.


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