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Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Печать: 2155-014X
ISSN Онлайн: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v8.i3.60
pages 253-260

Analysis of Quantal Characteristics of GABA Release during Short-Term Depression and Facilitation of Synaptic Transmission

Oksana P. Kolesnyk
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Svetlana A. Fedulova
O. O. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Mycola S. Veselovsky
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

Краткое описание

Changes in amplitudes of evoked paired inhibitory postsynaptic currents (eIPSCs) from rat cultured hippocampal neurons were studied using whole-cell patch-clamp technique and local extracellular electrical paired pulse stimulation of a single presynaptic axon. Paired pulse depression (PPD) and paired pulse facilitation (PPF) were observed in 43 tested neurons. According to the analysis of the coefficient of variation (CV) of eIPSCs, the CV of the second response during depression was significantly larger than that of the first one by 57% (n = 26), while it was smaller by 27% (n = 17) during facilitation. Thus, just presynaptic mechanisms underly short-term depression and facilitation. We also estimated quantal parameters assuming that a transmitter release is reasonably described by a binomial distribution. We have found that under our experimental conditions there were no significant changes in GABA release probability during PPD and PPF. The values of mean quantal content and mean number of sites of transmitter release were the same for the first stimulus in pair, and they were equal 9.7 in depression and 5 in facilitation of synaptic transmission. The values for the second stimulus decreased by 28 and 26% during PPD compared to the initial values, and they increased by 14 and 11%, respectively during PPF. Thus, there is significant difference in quantal parameters characterizing GABA release during short-term depression and facilitation of synaptic transmission.


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