Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Печать: 2155-014X
ISSN Онлайн: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v2.i1.10
pages 1-13

Characteristic of Sensory Neurotransmission in the Co-Culture of Both the Rat Dorsal Root Ganglion and the Rat Dorsal Horn Spinal Cord Neurons

Maria S. Shipshina
Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv
Mycola S. Veselovsky
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

Краткое описание

We examined properties of chemical neurotransmission at the level of primary afferent inputs into spinal cord with the new easy-to-use in vitro model of contiguously-cultivated dissociated both the dorsal root ganglion neurons (DRG) and the dorsal horn spinal cord neurons (DHSC) from newborn rats. The results of our studies showed presence of excitatory and inhibitory DRG neurons synapses on the cells of DHSC. The excitatory afferent signaling in such synapses was mediated by presynaptic release of glutamate and a following activation of both NMDA- and non-NMDA-receptor subclasses. In these cases the activation of non-NMDA- receptors makes a main contribution to realization excitatory postsynaptic effects. Either glycine- or GABA-ergic DRG neurons were involved in transmission of inhibitory signals to the DHSC neurons. However, in vast majority of examined neuronal pairs the inhibitory synaptic transmission was mediated by presynaptic release of glycine. As distinct from previous similar methods proposed here in vitro model of co-culture both the DRG and the DHSC neurons allows using a modern technical approaches to examination of the transmission of somatosensory information from the periphery to the CNS to the best advantage. The described model could be acceptable for detailed studding of specific properties of primary afferent synapses.

Articles with similar content:

Acetylcholine Release from the Central Nervous System: A 50-Year Retrospective
Critical Reviews™ in Neurobiology, Vol.17, 2005, issue 3-4
John W. Phillis
The Mouse Cerebellar Cortex in Organotypic Slice Cultures: An In Vitro Model to Analyze the Consequences of Mutations and Pathologies on Neuronal Survival, Development, and Function
Critical Reviews™ in Neurobiology, Vol.18, 2006, issue 1-2
Bernard Poulain, Jean-Louis Bossu, Jean-Luc Dupont, Huguette Beekenkamp, Etienne Lonchamp
Laminar Distribution of the Active Spinal Neurons during the Feeding-Related Stereotyped Movements in Rat
International Journal of Physiology and Pathophysiology, Vol.2, 2011, issue 2
Olexandr V. Dovgan', Andriy V. Maznychenko, Volodymyr A. Maisky, Oleh V. Vlasenko, Olexandr I. Pilyavskii
Oscillatory Circuits Underlying Locomotor Networks in the Rat Spinal Cord
Critical Reviews™ in Neurobiology, Vol.18, 2006, issue 1-2
Andrea Nistri, Giuliano Taccola
Neurotropic and Trophic Action of Lion's Mane Mushroom Hericium erinaceus (Bull.: Fr.) Pers. (Aphyllophoromycetideae) Extracts on Nerve Cells in Vitro
International Journal of Medicinal Mushrooms, Vol.9, 2007, issue 1
Mykhaylo Moldavan, Burkhard Kirchhoff, Paola Pedarzani, Andriy P. Grygansky, Olena V. Kolotushkina, Galina G. Skibo