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International Journal of Physiology and Pathophysiology
Главный редактор: Vadim F. Sagach (open in a new tab)

Выходит 4 номеров в год

ISSN Печать: 2155-014X

ISSN Онлайн: 2155-0158

SJR: 0.116

Pioglitazone, an Activator of Peroxisome Proliferator-Activated Receptor (PPAR-γ), Reduces the Expression of KB Nuclear Transcription Factor and Inhibits Apoptosis in Peripheral Blood Mononuclear Cells in Vitro

Том 3, Выпуск 4, 2012, pp. 371-377
DOI: 10.1615/IntJPhysPathophys.v3.i4.90
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Краткое описание

To examine the impact of PPAR-y activator on the expression of kB nuclear transcription factor (NF-kB) and apoptosis, the peripheral blood mononuclear cells (PBMC) were incubated in the presence of pioglitazone at a final concentration of 10, 30, and 100mmol/l. An expression of NF-kB in CD64+-cells and apoptosis were determined by flow cytofluorometry using appropriate monoclonal antibodies. The development of apoptosis was evaluated by using annexin V and propidium iodide. Activation of PPAR-γ led to a reduction of both a number of CD64+-cells and maximum levels of the receptor expression, as well as a dose-dependent decrease in the NF-kB concentration in the PBMC suspension and CD64+-cells. Analyzing apoptosis, we observed a reduction of apoptotic cells (AnV+ PI+-cells). The obtained results indicate that PPAR-γ activation is involved in the regulation of apoptosis in the PBMC and expression of NF-kB transcription factor, testifying that PPAR-γ is involved in the control of NF-kB activity, playing a leading role in the realization of inflammation, oncogenesis, and so on.

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