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International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v8.i1.30
pages 31-38

Comparative Study of Lectin Activity of Higher Basidiomycetes

Nona A. Mikiashvili
Institute of Evolution, University of Haifa, Israel; Iv. Javakhishvili State University of Tbilisi, Department of Biochemistry and Biotechnology, Georgia; Mushroom Biology & Fungal Biotechnology Laboratory, North Carolina A&T State University, USA
Vladimir I. Elisashvili
Animal Husbandry and Feed Production Institute of Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia
Solomon P. Wasser
N.G. Kholodny Institute of Botany, NAS of Ukraine, 2 Tereshchenkovskaya Str., Kiev 01004, Ukraine; International Center for Cryptogamic Plants and Fungi, Institute of Evolution, University of Haifa, Mount Carmel, Haifa, Israel
Eviatar D. Nevo
Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, Institute of Evolution, University of Haifa, 199 Abba Khousi Ave., Mt. Carmel, Haifa 3498838, Israel

Краткое описание

Lectin activity of extracts from medicinal and edible mushrooms fruiting bodies (13 species) as well as from mycelia grown on liquid (6 species, 9 strains), and malt extract agar (11 species, 23 strains) media was evaluated for the first time using a hemagglutination (HA) with rabbit erythrocytes. It has been proved that the capability to synthesize lectins is widespread among higher Basidiomycetes, and HA activity is not only species- but also strain-dependent. Tremendous lectin HA titer was revealed in fruiting body extracts from Agaricus pilatianus and Coprinus comatus and comparatively high HA activity was revealed in Cerrena unicolor and Ganoderma ramnosissimum mycelial biomass. G. ramnosissimum strain 162 showed the highest among all fungi tested specifically for HA activity (64512 U/mg). The HA activity of extracts was inhibited with a large variety of carbohydrates. Lactose was the most potent inhibitor of fruiting body extracts from Agaricus pilatianus, Corpinus comatus, C. micaceus, Macrolepiota rachodes, and Tricholoma fractum, while lectins from Amanita ovoidea, Lepista nuda, and Melanoleuca brevipes exerted the highest specificity for galactose. Glucose appeared to be an exceptionally potent inhibitor of HA activity of fruiting body extracts from Leucoagaricus leucothitus with a minimal inhibition concentration of 0.39 mM. Lactose inhibited lectin activity of Coprinus comatus at a concentration of 0.78 mM while a much higher concentration of the same sugar (12.5 mM) was needed to prevent HA by fruiting body extracts from C. micaceus. Liquid culture mycelia extracts from Cerrena unicolor and Trametes versicolor were galactose specific while Ganoderma lucidum and G. ramnosissmum showed specificity for arabinose and GlcNAc, respectively.


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