Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v6.i1.50
8 pages

Immune-Competent Polysaccharides from the Submerged Cultured Mycelium of Culinary—Medicinal Mushroom Lentinus strigellus Berk. et Curt. (Agaricomycetideae)

Yuexin Lin
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China
Ping Lai
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China
Yanyan Huang
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China
Hualing Xie
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China

Краткое описание

Two main fractions of polysaccharide SL-I and SL-II-b were purified from submerged cultured mycelium of culinary—medicinal mushroom Lentinus strigellus using hot water extraction, ethanol precipitation, DEAE-cellulose chromatography, and Sephadex G-100 filtration. Polysaccharide analysis showed that SL-I is a protein-free polysaccharide with 1.15 × 104 Da molecular weight and +63.33° optical rotation, while SL-II-b is a protein-conjugated polysaccharide with 3.88 × 103 Da molecular weight and +73.67° optical rotation. The sugar constituents of polysaccharide analysis by GC showed that both fractions are heteropolysaccharides. SL-I consists of six monosaccharides, and SL-II-b consists of three monosaccharides. IR and 1H-NMR, 13C-NMR spectra further indicated that LS-I and LS-II-b are heteropolysaccharides with a(1®2),(1®4) glucosidic bonds (LS-I), and a(1®2),(1®3),(1®4) glucosidic bonds (LS-II-b). The immune-competent examination showed that both polysaccharides could increase the spleen index, carbon clearance index, phagocytic index, and white blood cell levels of CY-treated mice.


Articles with similar content:

Fractionation and Characterization of Water-Soluble Polysaccharides from Culinary-Medicinal Mushroom, Agaricus blаzеi Murrill (Agaricomycetideae) Fruit Body
International Journal of Medicinal Mushrooms, Vol.4, 2002, issue 4
Ye Zhuqiu, Huang Yanyan, Xie Hualing, Lin Yuexin
Extraction of Conformationally Stable (1®6)-Branched (1®3)-b-Glucans from Premixed Edible Mushroom Powders by Cold Alkaline Solution
International Journal of Medicinal Mushrooms, Vol.4, 2002, issue 3
Manabu Kanai, Yoshiyuki Adachi, Toshiro Yadomae, Masaji Sawai, Susumu Matsui
Macrophages Stimulated by Polysaccharide Purified from Agaricus brasiliensis S.Wasser et al. (Agaricomycetideae) Enhance mRNA Expression of Th1 Cytokine Including IL-12 and 18
International Journal of Medicinal Mushrooms, Vol.5, 2003, issue 4
Nobuhide Fujitake, Sachiko Kawakami, Masashi Mizuno
Structure and Antitumor Activity of 1,3-b-Glucan from Cultivated Fruit Bodies of Culinary–Medicinal Mushroom Hypsizygus marmoreus (Peck) Bigel. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.5, 2003, issue 3
Shigeyuki Goto, Naohito Ohno, Masuro Motoi
Medium Selection for Exopolysaccharide and Biomass Production in Submerged Cultures of Culinary-Medicinal Mushrooms from Turkey
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 1
Mustafa Yamac, Meltem Kizilcik