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Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.1080
pages 478-479

Cytotoxic Activities of Funalia trogii (Berk.) Bond. Et. Singer ATCC 200800 Bioactive Extract on HeLa Cells and Fibroblast Cells

Ali Unyayar
Department of Environmental Engineering, Mersin University, Mersin 33343, Turkey
Murat Demirbilek
Department of Bioengineering Science Institute of Hacettepe University Ankara, Turkey
Melisa Turkoglu
Department of Bioengineering Science Institute of Hacettepe University Ankara, Turkey
Mehmet A. Mazmanci
Department of Environmental Engineering, Mersin University, Mersin 33343, Turkey
Emrah A. Erkurt
Department of Environmental Engineering, Mersin University, Mersin 33343, Turkey
Serpil Unyayar
Department of Biology, Mersin University, Mersin, Turkey
Ozlem Cekic
Department of Biology, Mersin University, Mersin, Turkey
Ayla Celik
Department of Biology, Mersin University, Mersin, Turkey
Hatice Atacag
Department of Environmental Engineering, Mersin University, Mersin 33343, Turkey

Краткое описание

This study demonstrated the cytotoxic and cell growth inhibitory effect of a bioactive extract of Funalia trogii (Berk.) Bond. et Singer, which was grown in solid-state fermentation at room temperature, on He-La and fibroblast and the mitotic index of lymphocytes. The effect demonstrated by the bioactive extract of F. trogii on laccase, peroxidase, SOD, ascorbate peroxidase, catalase, and glutation peroxidase is shown in Table 1.
A study on the possible cytotoxic and cell growth inhibitory effects of the bioactive extracts of two white-rot fungi showed that these extracts had significant cytotoxic and antiproliferative properties on the HeLa cancer cell line. Hence, the aim of this study was to examine the in vitro cytotoxic activities of standardized aqueous bioactive extracts prepared from Funalia trogii on HeLa and fibroblast cell lines using a MTT (3-[4,5-Dimetiltiazol-2-]-2-5-difeniltetrazolium bromide) cytotoxicity assay. F. trogii solutions were found to exhibit cytotoxic effects on HeLa cell lines. Based on the data, it was found that toxicity ratios of 0.05 μL of F. trogii extract solutions were 71.5%. Furthermore, F. trogii extract solutions were also cytotoxic on fibroblast cell lines.
Analysis of the data indicated that the toxicity ratio of 0.05 μL of F. trogii solutions was 51.3%. These results showed that the extracts had a substantial cytotoxic action on HeLa cell lines but less on fibroblast cells. In separate experiments the mitotic index reached nearly the same value at 4 μg/mL MMC, 75 μL concentrations of non-heated fungal extract. Therefore, in order to compare the mutagenic potential of fungal extract and MMC, we used concentration values in SCE analysis. Table 2 represents the SCE frequency of cultures treated with non-heated fungal extract, positive control, and negative controls. A significant induction of SCE was observed in cultured lymphocytes treated with MMC (4 μg/mL) compared with non-heated fungal extract and negative control (p < 0.001). There was no significant difference between negative control and non-heated fungal extract (p > 0.05, p = 0.73). In conclusion, we did not observe any genotoxic effect.
In bioactive extracts of fungi, we revealed the determination of enzyme or enzymes responsible for cytotoxic effect on HeLa cell line. As a result, antitumor activity was shown by two enzymes—laccase and peroxidase—produced by fungi. Bioactive extracts have natural quinone substances from lignin by production of peroxidase and laccase. These enzymes acted more selectively on HeLa cells, arresting the cell in the G-phase of the cell cycle and inducing apoptosis. The basis of our work was that bioreductive activation was also a highly specific delivery mechanism for targeting a variety of processes and was important for tumor growth. Thus, bioreduction of a quinone in the hypoxic region of a tumor would result in the formation of an intermediate semiquinone or hydroquinone (depending on 1-e or 2-e reduction). Hence, we proved that the covalent bond in quinone is meta-bolically stable, the effector quinone substance is only released within the hypoxic regions of tumors, and the desired large differential between the effects of the quinone is attained. We are able to produce the quinones naturally, which can act as excellent subtrates for NAD(P) H-quinone oxidoreductase (DT-diaphorase) and thus target tumors rich in this enzyme, thereby providing cytotoxic activation by a hypoxia independent mechanism.
This study provides evidence for in vitro antitumor activity of a bioactive extract from F. trogii. Therefore, upon in vivo data, which will follow this study, it may become a promising cytotoxic product for treatment of various types of cancer.


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