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International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.680
427 pages

Cytotoxic Activities of Ergosta-4, 6, 8 (14), 22-tetraen-3-one from the Sclerotia of Grifola umbellata (Pers.) Pilát

Wi Young Lee
Division of Biotechnology, Korea Forest Research Institute, Suwon 441-350, S. Korea
Hak Ju Lee
Div. Wood Chemistry & Microbiology, Korea Forest Research Institute, Seoul 130-712, S. Korea
Youngki Park
Division of Biotechnology, Korea Forest Research Institute, Suwon 441-350, S. Korea
Jin Kwon Ahn
Division of Biotechnology, Korea Forest Research Institute, Suwon 441-350, S. Korea
Kang Hyeon Ka
Div. Wood Chemistry & Microbiology, Korea Forest Research Institute, Seoul 130-712, S. Korea

Краткое описание

Grifola umbellata (also known as Polyporus umbellatus) is a mushroom that is used as a diuretic in Chinese medicine. Herein, we report on the isolation and identification of a cytotoxic compound from the sclerotia of G. umbellata.
For the isolation of the compound, a hexane soluble fraction of the sclerotia of G. umbellata was subjected to column chromatography on silica gel and/or Sephadex LH-20 column eluted with organic solvents. The structure of the isolated compound was elucidated using IR, MS, 1H-, and 13C-NMR spectra, and the structure of the compound was determined as ergosta-4, 6, 8 (14), 22-tetraen-3-one (erogone).
Cytotoxic activities of erogone compared to human cancer cell lines, HT-29 (colon cancer), HeLa 229 (cervix cancer), Hep3B (liver cancer), and AGS (stomach cancer) were compared using the XTT assay kit. Ergone inhibited all cell lines as the dose was increased. In the case of Hep3B and HT-29 cell lines, maximal cytotoxic activities of erogone were achieved at the concentrations of 10 and 15 μ/mL, respectively. However, the cytotoxic activities of erogone compared to HeLa 229 and AGS were much weaker than those of Hep3B and HT-29 cell lines. Values of 50% inhibitory concentrations (IC50) of erogone against Hep3B, HT-29, HeLa 229, and AGS were 5, 7.2, 26.3, and 22 μ/mL, respectively.
Cytotoxic activities of erogone against various tumor cell lines were evaluated in this study for the first time. Therefore, other mushrooms were also examined for the presence of the compound. In the present study, we measured the content of erogone in eight mushrooms—Grifola umbellata, Lentinus edodes (Berk.) Singer, Ganoderma applanatum (Pers.:Wallr.) Pat., Tricholoma matsutake (S.Ito et S.Imai) Singer, Sarcodon aspratus (Berk.) S.Ito, Ramaria botrytis (Pers.) Ricken, Pleurotus eryngii (DC.:Fr.) Quél., and Sparassis crispa (Wulf.)Fr.—and four mycelia—Grifola umbellata, Lentinus edodes, Ganoderma applanatum, and Tricholoma matsutake—using HPLC. The contents of erogone in mushroom and mycelium were in the range of 4.8−29.0 μ/g and 15.5−38.0 μ/g, respectively. Among mushrooms and mycelia tested, the mycelia of Grifola umbellata and Tricholoma matsutake had the highest amounts of erogone (38.0 μ/g).


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