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ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344
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Isolation and Purification of Polysaccharides with Anti-tumor Activity from Pholiota adiposa (Batsch) P. Kumm. (Higher Basidiomycetes)
Краткое описание
Pholiota adiposa is a mushroom with excellent medicinal and nutritional properties. After culture in fermentation medium, Ph. Adiposa mycelia were filtered, lyophilized, and powdered. A crude polysaccharide (PAP) of Ph. Adiposa was prepared from the mycelial powder with hot water, centrifuged, and the resulting supernatant lyophilized. PAP was fractionated by 30%, 60%, and 80% ethanol precipitation steps to yield PAP30, PAP60, and PAP80. Subsequently PAP30-1 and PAP30-2, PAP60-1 and PAP60-2, and PAP80-1 and PAP80-2 were isolated from PAP30, PAP60, and PAP80, respectively, by ion-exchange chromatography on a DEAE-Sepharose column. Polysaccharide content increased from 43.8% in PAP to 50.54%~73.19% in PAP30-1~PAP80-2. The protein content was 4.92% at minimum in these polysaccharide products. In order to identify the chemical composition, the six polysaccharides (PAP30-1, PAP30-2, PAP60-1, PAP60-2, PAP80-1, and PAP80-2) were further purified by gel filtration on Sephacryl S-100-500. Finally, three water-soluble polysaccharides (PAP30-2a, PAP60-2b, and PAP80-2a) were obtained. HPLC analysis revealed that PAP30-2a, PAP60-2b, and PAP80-2a exhibited a molecular weight of 6.6 × 105 Da, 8.4 × 103 Da, and 3.5 × 103 Da, respectively. The glucose content in PAP80-2a, PAP60-2b, and PAP30-2a was 57.8%, 72.7%, and 68.9%, respectively. PAP30-2a, PAP60-2b, and PAP80-2a demonstrated significant differences in anti-tumor activity in mice. PAP80-2a is the optimal bioactive constituent with anti-tumor and T-lymphocyte proliferation stimulating effects.
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Villares Ana, Mateo-Vivaracho Laura, Guillamón Eva, Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms, Agriculture, 2, 4, 2012. Crossref
-
汪 治云, Progress in the Study of the Biological Activities and Extraction and Purification of Edible Mushroom Polysaccharides, Advances in Microbiology, 04, 03, 2015. Crossref
-
Friedman Mendel, Mushroom Polysaccharides: Chemistry and Antiobesity, Antidiabetes, Anticancer, and Antibiotic Properties in Cells, Rodents, and Humans, Foods, 5, 4, 2016. Crossref
-
Yuan Xianghe, Zhu Mengjuan, Tian Guoting, Zhao Yongchang, Zhao Liyan, Ng Tzi Bun, Wang Hexiang, Biochemical characteristics of a novel protease from the basidiomyceteAmanita virgineoides, Biotechnology and Applied Biochemistry, 64, 4, 2017. Crossref
-
Teixeira da Silva Jaime A., Ng Tzi Bun, The medicinal and pharmaceutical importance of Dendrobium species, Applied Microbiology and Biotechnology, 101, 6, 2017. Crossref
-
Zou Yajie, Du Fang, Hu Qingxiu, Wang Hexiang, The structural characterization of a polysaccharide exhibiting antitumor effect from Pholiota adiposa mycelia, Scientific Reports, 9, 1, 2019. Crossref
-
Wu Fang, Zhou Li-Wei, Yang Zhu-Liang, Bau Tolgor, Li Tai-Hui, Dai Yu-Cheng, Resource diversity of Chinese macrofungi: edible, medicinal and poisonous species, Fungal Diversity, 98, 1, 2019. Crossref
-
Lee Jun Won, Park Myung Soo, Park Ji-Hyun, Cho Yoonhee, Kim Changmu, Kim Chang Sun, Jo Jong Won, Lim Young Woon, Taxonomic Study of the Genus Pholiota (Strophariaceae, Basidiomycota) in Korea, Mycobiology, 48, 6, 2020. Crossref
-
Choudhary Sunil, Characterization and applications of mushroom exopolysaccharides, in New and Future Developments in Microbial Biotechnology and Bioengineering, 2020. Crossref
-
Rong Chengbo, Song Shuang, Yang Li, Pan Xuejiao, Liu Yu, Wang Shouxian, Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa, Ciência e Agrotecnologia, 45, 2021. Crossref
-
Kothari Damini, Patel Seema, Kim Soo-Ki, Anticancer and other therapeutic relevance of mushroom polysaccharides: A holistic appraisal, Biomedicine & Pharmacotherapy, 105, 2018. Crossref
-
Chun Sechul, Gopal Judy, Muthu Manikandan, Antioxidant Activity of Mushroom Extracts/Polysaccharides—Their Antiviral Properties and Plausible AntiCOVID-19 Properties, Antioxidants, 10, 12, 2021. Crossref
-
Wu Chenlu L, Xu Lili L, Peng Jing, Zhang Danhua H, Al-MPS Obstructs EMT in Breast Cancer by Inhibiting Lipid Metabolism via miR-215-5p/SREBP1, Endocrinology, 163, 5, 2022. Crossref
-
Yang Zhongwei, Liu Zijing, Xu Jie, Zhu Junmo, Pu Youwei, Bao Yixi, Study on the physicochemical properties and immunomodulatory anti-tumor effect of the Pholiota adiposa polysaccharide, Food & Function, 13, 9, 2022. Crossref