Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Том 22, 2020 Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v14.i3.40
pages 271-284

Isolation and Purification of Polysaccharides with Anti-tumor Activity from Pholiota adiposa (Batsch) P. Kumm. (Higher Basidiomycetes)

Qingxiu Hu
Institute of agricultral source and regional planning research, Chinese Academy of Agricultural Sciences, Beijing 100081, China ; State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193
Hexiang Wang
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China
Tzi Bun Ng
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

Краткое описание

Pholiota adiposa is a mushroom with excellent medicinal and nutritional properties. After culture in fermentation medium, Ph. Adiposa mycelia were filtered, lyophilized, and powdered. A crude polysaccharide (PAP) of Ph. Adiposa was prepared from the mycelial powder with hot water, centrifuged, and the resulting supernatant lyophilized. PAP was fractionated by 30%, 60%, and 80% ethanol precipitation steps to yield PAP30, PAP60, and PAP80. Subsequently PAP30-1 and PAP30-2, PAP60-1 and PAP60-2, and PAP80-1 and PAP80-2 were isolated from PAP30, PAP60, and PAP80, respectively, by ion-exchange chromatography on a DEAE-Sepharose column. Polysaccharide content increased from 43.8% in PAP to 50.54%~73.19% in PAP30-1~PAP80-2. The protein content was 4.92% at minimum in these polysaccharide products. In order to identify the chemical composition, the six polysaccharides (PAP30-1, PAP30-2, PAP60-1, PAP60-2, PAP80-1, and PAP80-2) were further purified by gel filtration on Sephacryl S-100-500. Finally, three water-soluble polysaccharides (PAP30-2a, PAP60-2b, and PAP80-2a) were obtained. HPLC analysis revealed that PAP30-2a, PAP60-2b, and PAP80-2a exhibited a molecular weight of 6.6 × 105 Da, 8.4 × 103 Da, and 3.5 × 103 Da, respectively. The glucose content in PAP80-2a, PAP60-2b, and PAP30-2a was 57.8%, 72.7%, and 68.9%, respectively. PAP30-2a, PAP60-2b, and PAP80-2a demonstrated significant differences in anti-tumor activity in mice. PAP80-2a is the optimal bioactive constituent with anti-tumor and T-lymphocyte proliferation stimulating effects.

Articles with similar content:

Characterization and Antiproliferative Effect of Novel Acid Polysaccharides from the Spent Substrate of Shiitake Culinary-Medicinal Mushroom Lentinus edodes (Agaricomycetes) Cultivation
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 5
Peixin He, Wei Liu, Yong Zhang, Chunping Xu, Wei Huang
Triterpenes and Sterols from Ganoderma australe (Fr.) Pat. (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.2, 2000, issue 4
Nelson Biacchi, Jr., Alexandra Lehmkuhl Gerber, Artur Smania, Jr., Franco Delie Monache, Elza de Fatima Albino Smania
Isolation and Characterization of Lectin from the Artist's Conk Medicinal Mushroom, Ganoderma applanatum (Agaricomycetes), and Evaluation of Its Antiproliferative Activity in HT-29 Colon Cancer Cells
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 8
Ashok Kumar Pandurangan, Norhaizan Mohd. Esa, Sekar Kumaran, Ramalingam Shenbhagaraman
Effects of Various Treatments on Extraction of the Main Bioactive Components and Determination of Biological Activity of Extracts from the Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 9
Chaomin Yin, Hong Gao, Xiuzhi Fan, De-Fang Shi, Zhe Fan
Characterization of Exopolysaccharide Produced by Phellinus vaninii (Agaricomycetes) and Antioxidant Potential for Meat Batter
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 5
Yanchao Yao, Xuewei Jia, Junguang Li, Yanhong Bai