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Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 22, 2020 Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v16.i5.30
pages 431-447

Effects of Medium Components and Culture Conditions on Mycelial Biomass and the Production of Bioactive Ingredients in Submerged Culture of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus

Jian-Zhi Chen
Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, Taiwan
Hui-Chen Lo
Department of Natural Sciences, Fu Jen Catholic University, #510, JhongZheng Rd., Xinzhuang District, New Taipei City, 24205, Taiwan, ROC
Fang-Yi Lin
Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan
Shih-Liang Chang
School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Changhua, Taiwan
Chang-Wei Hsieh
Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.)
Zeng-Chin Liang
Department of Bioresources, Da-Yeh University, Changhua County 515, Taiwan
Wai-Jane Ho
Department of Medicinal Botanicals and Healthcare, Department of Bioresources, Da-Yeh University, Datsun, Changhua, 51591 Taiwan
Tai-Hao Hsu
Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.); Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.)

Краткое описание

The optimal culture conditions were investigated to maximize the production of mycelial biomass and bioactive ingredients in submerged cultivation of Xylaria nigripes, a Chinese medicinal fungus. The one-factor-at-a-time method was used to explore the effects of medium components, including carbon, nitrogen, mineral sources, and initial pH of the medium and environmental factors, such as culture temperature and rotation speed, on mycelial growth and production of bioactive ingredients. The results indicated that the optimal culture temperature and rotation speed were 25°C and 100 rpm in a medium with 20 g fructose, 6 g yeast extract, and 2 g magnesiun sulfate heptahydrate as carbon, nitrogen, and mineral sources, respectively, in 1 L distilled water with an initial medium pH of 5.5. With optimal medium components and conditions of cultivation, the maximal production of mycelial biomass was 6.64 ± 0.88 g/L, with maximal production of bioactive ingredients such as extracellular polysaccharides (2.36 ± 0.18 mg/mL), intracellular polysaccharides (2.38 ± 0.07 mg/g), adenosine (43.27 ± 2.37 mg/g), total polyphenols (36.57 ± 1.36 mg/g), and triterpenoids (31.29 ± 1.17 mg/g) in a shake flask culture. These results suggest that different bioactive ingredients including intracellular polysaccharides, adenosine, total polyphenols and triterpenoids in mycelia and extracellular polysaccharides in broth can be obtained from one simple medium for submerged cultivation of X. nigripes.