Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 22, 2020 Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2020033335
pages 115-124

Antibacterial Activity of Fruiting Body Extracts from Culinary-Medicinal Winter Mushroom, Flammulina velutipes (Agaricomycetes) against Oral Pathogen Streptococcus mutans

Chen Cui
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Dept. of Endodontic Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, P.R. China
Hui Xu
shenyang pharmaceutical university; Department of Food Quality and Safety/National R&D Center For Chinese Herbal Medicine Processing, China Pharmaceutical University, Nanjing, P.R. China
Hua Yang
State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, P.R. China
Rui Wang
State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, P.R. China
Yu Xiang
Department of Food Quality and Safety, School of Engineering, China Pharmaceutical University, Nanjing, P.R. China
Bingxuan Li
Department of Food Quality and Safety, School of Engineering, China Pharmaceutical University, Nanjing, P.R. China
Xuedong Zhou
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Dept. of Endodontic Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, P.R. China
Ruijie Huang
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Dept. of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, P.R. China
Shujie Cheng
Department of Food Quality and Safety/National R&D Center For Chinese Herbal Medicine Processing, China Pharmaceutical University, Nanjing, P.R. China

Краткое описание

The edible medicinal mushroom Flammulina velutipes (enokitake) has many applications as food and medicine, but its application in dentistry is unknown. This study aims to investigate the inhibitory effect of fruiting body extracts from F. velutipes on the growth and adhesion of Streptococcus mutans, the main cause of human caries, in vitro. Of the four extracts (named TG01 from water, TG02 from 95% ethanol, TG03 from 50% ethanol, and TG04 from ethyl acetate), TG03 had significant antibacterial activity (MIC = 10 mg/mL; MBC = 20 mg/mL). Planktonic growth and biofilm formation in S. mutans was repressed by TG03 at 5 mg/mL and above. Meanwhile, cytotoxicity analysis showed that TG03 was not toxic to human oral keratinocyte cells. HPLC-QQQ-MS analysis showed that the TG03 extract contained a large amount of arabitol, a sucrose substitute that reduces the development of caries. Thus, F. velutipes extracts can effectively inhibit the growth of the oral pathogen S. mutans without cytotoxicity against human oral keratinocytes. Therefore, F. velutipes is a good candidate for the development of oral hygiene agents to control dental caries.

ЛИТЕРАТУРА

  1. Selwitz RH, Ismail AI, Pitts NB. Dental Caries. Lancet. 2007;369(9555):51-59.

  2. Russell RR. How has genomics altered our view of caries microbiology? Caries Res. 2008;42(5):319-327.

  3. Bowen WH, Koo H. Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res. 2011;45(1):69-86.

  4. Hong SW, Baik JE, Kang SS, Yun CH, Seo DG, Han SH. Lipoteichoic acid of Streptococcus mutans interacts with tolllike receptor 2 through the lipid moiety for induction of inflammatory mediators in murine macrophages. Mol Immunol. 2014;57(2):284-91.

  5. Loman AA, Ju LK. Inhibitory effects of arabitol on caries-associated microbiologic parameters of oral Streptococci and Lactobacilli. Arch Oral Biol. 2015;60(12):1721-28.

  6. Chaffee BW, Cheng J, Featherstone JD. Non-operative anticaries agents and dental caries increment among adults at high caries risk: a retrospective cohort study. BMC Oral Health. 2015;15(1):111.

  7. Wang C, Liu J, Su D. Preliminary screen of high cariogenicity Streptococcus mutans strains isolated from clinical specimens [in Chinese]. West China J Stomatol. 2013;31(2):136-40.

  8. Marsh PD. Are dental diseases examples of ecological catastrophes? Microbiology. 2003;149(Pt 2):279-94.

  9. Huang R, Li M, Gregory RL. Effect of nicotine on growth and metabolism of Streptococcus mutans. Eur J Oral Sci. 2012;120(4):319-25.

  10. Hall-Stoodley L, Costerton JW, Stoodley P. Bacterial biofilms: from the natural environment to infectious diseases. Nat Rev Microbiol. 2004;2(2):95-108.

  11. Marsh PD, Moter A, Devine DA. Dental plaque biofilms: communities, conflict and control. Periodontal 2000. 2011;55(1):16-35.

  12. Stewart PS. Biophysics of biofilm infection. Pathog Dis. 2014;70(3):212-18.

  13. Wasser SP. Medicinal mushrooms in human clinical studies. Part I. Anticancer, oncoimmunological, and immunomodulatory activities: a review. Int J Med Mushrooms. 2017;19:279-317.

  14. Wasser SP. Medicinal mushroom science: history, current status, future trends, and unsolved problems. Int J Med Mushrooms. 2010;12(1):1-16.

  15. Chang ST, Wasser SP. Current and future research trends in agricultural and biomedical applications of medicinal mushrooms and mushroom products (review). Int J Med Mushrooms. 2018;20(11):1034-48.

  16. Lingstrom P, Zaura E, Hassan H, Buijs MJ, Hedelin P, Pratten J, Spratt D, Daglia M, Karbowiak A, Signoretto C, Rosema M, van der Weijden F, Wilson M. The anticaries effect of a food extract (shiitake) in a short-term clinical study. J Biomed Biotechnol. 2012;2012:217164.

  17. Papetti A, Signoretto C, Spratt DA, Pratten J, Lingstrom P, Zaura E, Ofek I, Wilson M, Pruzzo C, Gazzani G. Components in Lentinus edodes mushroom with antibiofilm activity directed against bacteria involved in caries and gingivitis. Food Funct. 2018;9(6):3489-99.

  18. Signoretto C, Marchi A, Bertoncelli A, Burlacchini G, Papetti A, Pruzzo C, Zaura E, Lingstrom P, Ofek I, Pratten J, Spratt DA, Wilson M, Canepari P. The anti-adhesive mode of action of a purified mushroom (Lentinus edodes) extract with anticaries and antigingivitis properties in two oral bacterial phatogens. BMC Complement Altern M. 2014;14:75.

  19. Avinash J, Vinay S, Jha K, Das D, Goutham BS, Kumar G. The unexplored anticaries potential of shiitake mushroom. Pharmacogn Rev. 2016;10(20):100-4.

  20. Jia W, Feng J, Zhang JS, Lin CC, Wang WH, Chen HG. Structural characteristics of the novel polysaccharide FVPA1 from winter culinary-medicinal mushroom, Flammulina velutipes (Agaricomycetes), capable of enhancing natural killer cell activity against K562 Tumor Cells. Int J Med Mushrooms. 2017;19(6):535-46.

  21. Tsai SY, Huang EW, Lin CP. Compositional differences of the winter culinary-medicinal mushroom, Flammulina velutipes (Agaricomycetes), under three types of light conditions. Int J Med Mushrooms. 2017;19(3):267-76.

  22. Dong YR, Cheng SJ, Qi GH, Yang ZP, Yin SY, Chen GT. Antimicrobial and antioxidant activities of Flammulina velutipes polysaccharides and polysaccharide-iron(III) complex [corrected]. Carbohydr Polym. 2017;161:26-32.

  23. Su A, Yang W, Zhao L, Zhao L, Pei F, Yuan B, Zhong L, Ma G, Hu Q. Flammulina velutipes polysaccharides improve scopolamine-induced learning and memory impairment in mice by modulating gut microbiota composition. Food Funct. 2018;9(3):1424-32.

  24. Lien HM, Tseng CJ, Huang CL, Lin YT, Chen CC, Lai YY. Antimicrobial activity of Antrodia camphorata extracts against oral bacteria. PLoS One. 2014;9(8):e105286.

  25. Huang R, Li M, Ye M, Yang K, Xu X, Gregory RL. Effects of nicotine on Streptococcus gordonii growth, biofilm formation, and cell aggregation. Appl Environ Microbiol. 2014;80(23):7212-18.

  26. Kordowska-Wiater M, Kuzdralinski A, Czernecki T, Targonski Z, Frac M, Oszust K. The ability of a novel strain Scheffer-somyces (syn. Candida) shehatae isolated from rotten wood to produce arabitol. Pol J Microb. 2017;66(3):335-43.

  27. Loman AA, Ju LK. Inhibitory effects of arabitol on caries-associated microbiologic parameters of oral Streptococci and Lactobacilli. Arch Oral Biol. 2015;60(12):1721-28.

  28. Kitamoto Y, Gruen HE. Distribution of cellular carbohydrates during development of the mycelium and fruitbodies of Flammulina velutipes. Plant Physiol. 1976;58(4):485-91.

  29. Tereshina VM, Memorskaia AS. Adaptation of Flammulina velutipes to hypothermia in natural environment: the role of lipids and carbohydrates [in Russian]. Mikrobiologiia. 2005;74(3):329-34.

  30. Valm AM, Mark Welch JL, Rieken CW, Hasegawa Y, Sogin ML, Oldenbourg R, Dewhirst FE, Borisy GG. Systems-level analysis of microbial community organization through combinatorial labeling and spectral imaging. Proc Natl Acad Sci U S A. 2011;108(10):4152-57.

  31. Firestone AR, Schmid R, Muhlemann HR. Cariogenic effects of cooked wheat starch alone or with sucrose and frequency-controlled feedings in rats. Arch Oral Biol. 1982;27(9):759-63.

  32. Ribeiro CC, Tabchoury CP, Del Bel Cury AA, Tenuta LM, Rosalen PL, Cury JA. Effect of starch on the cariogenic potential of sucrose. Br J Nutr. 2005;94(1):44-50.

  33. Paes Leme AF, Koo H, Bellato CM, Bedi G, Cury JA. The role of sucrose in cariogenic dental biofilm formation-new insight. J Dent Res. 2006;85(10):878-87.


Articles with similar content:

Antimicrobial Activity of Extracts of the Oyster Culinary Medicinal Mushroom Pleurotus ostreatus (Higher Basidiomycetes) and Identification of a New Antimicrobial Compound
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 6
Ahmed M. Younis, Hussien H. El Shikh, Fang-Sheng Wu
Antimicrobial and Antitoxical Action of Edible and Medicinal Mushroom Pleurotus ostreatus (Jacq.: Fr.) Kumm. Extracts
International Journal of Medicinal Mushrooms, Vol.4, 2002, issue 2
Irina V. Tolstych, Olga V. Efremenkova, Valentina A. Zenkova, Olga V. Kamzolkina, Valeriy P. Gerasimenya, Tatyana A. Bogush
Tropical Milky White Mushroom, Calocybe indica (Agaricomycetes): An Effective Antimicrobial Agent Working in Synergism with Standard Antibiotics
International Journal of Medicinal Mushrooms, Vol.22, 2020, issue 4
Shweta Gupta, Jyoti Dubey, Arup Kumar Mitra, Suhana Datta, Ayoshi Paul, Payel Gupta
Evaluation of Anticholinesterase and Inflammation Inhibitory Activity of Medicinal Mushroom Phellinus pini (Basidiomycetes) Fruiting Bodies
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 11
Tae Soo Lee, Jae-hyuk Choi, Jae Kwang Kim, Trung Kien Nguyen, Kyung Hoan Im
Anti-Infective Properties of the Melanin-Glucan Complex Obtained from Medicinal Tinder Bracket Mushroom, Fomes fomentarius (L.: Fr.) Fr. (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 1
Galina V. Beketova, Alla B. Prilutckaya, Nataliya O. Savichuk, Petr G. Rytik, Igor I. Kucherov, Leontij F. Gorovoj, Alexandr I. Prilutsky, Olga F. Senyuk