Выходит 12 номеров в год
ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344
Indexed in
Anti-Inflammatory Activity and Bioactive Constituents of Cultivated Fruiting Bodies of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus
Краткое описание
Xylaria nigripes, also known as Wu Ling Shen, is popular for treating insomnia and trauma in traditional Chinese medicine. This study aimed to examine the anti-inflammatory activity and bioactive constituents of cultivated X. nigripes fruiting bodies in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Results showed that among the different extracts, the hexane fraction exhibited the best protection against cell toxicity induced by 1 μg/mL LPS and the strongest inhibitory effect on nitric oxide (NO) production. This fraction led to the isolation of 2 bioactive compounds (namely, XN-CP1 and XN-CP2), which were confirmed to be ergostarien-3β-ol and ergosterol peroxide, respectively. Although both XN-CP1 and XN-CP2 showed good inhibitory effects on NO, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and prostaglandin E2 production in LPS-stimulated macrophages, XN-CP2 was shown to have a stronger anti-inflammatory activity; this was further supported by its strong suppressive effects on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression and nuclear factor (NF)-κB activation. These results conclude that ergosterol peroxide (XN-CP2) could be the main bioactive compound contributing to the potent anti-inflammatory activity of X. nigripes, and its mechanism of action is mediated through inhibition of iNOS and COX-2 expression via the NF-κB signaling pathway.
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Wu Shu-Jing, Tung Yi-Jou, Ng Lean-Teik, Anti-diabetic effects of Grifola frondosa bioactive compound and its related molecular signaling pathways in palmitate-induced C2C12 cells, Journal of Ethnopharmacology, 260, 2020. Crossref
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Lai Ming-Nan, Hsu Hui-Chen, Ng Lean-Teik, Safety assessment of the standardized aqueous extract from solid-state cultured Xylaria nigripes (Wuling Shen) in rats, Clinical Phytoscience, 7, 1, 2021. Crossref
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Keekan K. K., Ranadive K. R., Naik P., Sendker J., Padmaraj S. R., Anti-Methicillin-Resistant S. aureus Activity of Fruiting Body and Mycelial Culture Extracts of Xylaria longipes Nitschke (Ascomycota), Pharmaceutical Chemistry Journal, 56, 7, 2022. Crossref