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International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v13.i2.50
pages 131-143

Anticancer Effects of Fraction Isolated from Fruiting Bodies of Chaga Medicinal Mushroom, Inonotus obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae): In Vitro Studies

Marta Kinga Lemieszek
Department of Medical Biology, Institute of Rural Health, Lublin, Poland
Ewa Langner
Department of Medical Biology, Institute of Agricultural Medicine; and Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Skіodowska University, Lublin, Poland
Jozef Kaczor
Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin
Martyna Kandefer-Szerszen
Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin
Bozena Sanecka
Department of Biochemistry and Biotechnology, Faculty of Chemistry, Rzeszów University of Technology, Al. Powstanców Warszawy 6, 35-959 Rzeszów
Witold Mazurkiewicz
Department of Organic Chemistry, Faculty of Chemistry, Rzeszów University of Technology, Al. Powstanców Warszawy 6, 35-959 Rzeszów
Wojciech Rzeski
Department of Medical Biology, Institute of Rural Health, Lublin, Poland; Department of Virology and Immunology, Maria Curie-Sklodowska University, Lublin, Poland

Краткое описание

The medicinal mushroom Chaga, Inonotus obliquus (Pers.:Fr.) Pilát (Hymenochaetaceae), has been used in folk medicine in Russia, Poland, and most of the Baltic countries, as a cleansing and disinfecting measure, and as decoctions for stomach diseases, intestinal worms, liver and heart ailments, and cancer treatment. Many reports have been published concerning the health promoting functions of this mushroom, including antibacterial, hepatoprotective, anti-inflammatory, antitumor, and antioxidant activities. The purpose of the present study was evaluation of in vitro anticancer activity of fraction IO4 isolated from I. obliquus. The effect on cell proliferation, motility and viability was assessed in a range of cancer and normal cells. Chaga fraction prepared from dried fruiting bodies was subjected to anticancer evaluation in human lung carcinoma (A549), colon adenocarcinoma (HT-29), and rat glioma (C6) cell cultures. Human skin fibroblasts (HSF), bovine aorta endothelial cells (BAEC), models of rat oligodendrocytes (OLN-93), hepatocytes (Fao), rat astroglia, and mouse neurons (P19) were applied to test toxicity in normal cells. The following methods were applied: tumor cell proliferation (MTT assay and BrdU assay), cytotoxicity (LDH assay), tumor cell motility (wound assay), tumor cell morphology (May-Grünwald-Giemsa staining), and death detection (ELISA). Chaga fraction elicited anticancer effects which were attributed to decreased tumor cell proliferation, motility and morphological changes induction. Of note is the fact that it produced no or low toxicity in tested normal cells. The data presented could open interesting paths for further investigations of fraction IO4 as a potential anticancer agent.


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