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International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 22, 2020 Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v4.i2.30
16 pages

Th1-Oriented Immunomodulating Activity of Gel-Forming Fungal (1®3)-Beta-Glucans

Naohito Ohno
Tokyo University of Pharmacy and Life Sciences School of Pharmacy, Lab Immunopharmacological and Microbiological Production, 1432-1 Horinouchi, Hachioji Tokyo, 192-0392, Japan
Takafumi Jinushi
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
Toshiro Yadomae
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

Краткое описание

The immunomodulating effects of various gel-forming (1®3)-beta-glucans on balancing helper T-cell activity were examined in a murine model. Plasma from mice injected with grifolan (GRN) from the edible and medicinal mushroom Grifola frondosa (Dicks.: Fr.) S. F. Gray [or alkaline-treated sonifilan (SPG-OH)] and trinitrophenyl ovalbumin (TNP-OVA) contained TNP-specific antibodies of both IgG1 (Th2-mediated) and IgG2a (Th1-mediated) isotypes. Administration of glucan (SSG) from Sclerotinia sclerotiorum (Lib.) de Bary IFO 9395 and TNP-OVA significantly augmented the synthesis of IgG2a antibodies, while the synthesis of IgG1 was reduced. Furthermore, intracellular cytokine staining showed a higher proportion of IFN-g-producing helper T cells in SSG-administered murine splenocytes. The highest IFN-g production was observed when adherent cells of mice administered TNP-OVA and SSG were cultured with TNP-primed lymphocytes. This effect of administration of SSG on IFN-g production was completely inhibited by addition of anti-IL-12 mAb. The content of the reduced form of glutathione in splenocytes was increased by SSG administration, suggesting type 1 immunoresponse. Depletion of lymphoid dendritic cell population from SSG-administered splenocytes reduced IFN-g production. In conclusion, our study showed that beta-glucans have various effects on the Th1- or Th2-dependent antibody subclasses and, in particular, that SSG induces the development of Th1 cells via the IL-12 pathway.