Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.211 5-летний Импакт фактор: 1.394 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v18.i12.80
pages 1131-1140

Downregulation of Ethylene Production Increases Mycelial Growth and Primordia Formation in the Button Culinary-Medicinal Mushroom, Agaricus bisporus (Agaricomycetes)

Chaohui Zhang
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China
Tao Huang
Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural University, Zhengzhou, P.R. China
Chaohui Shen
Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural University, Zhengzhou, P.R. China
Xiaoting Wang
Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural University, Zhengzhou, P.R. China
Yuancheng Qi
Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural University, Zhengzhou, P.R. China
Jinwen Shen
College of Life Sciences, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China
Andong Song
Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural University, Zhengzhou, P.R. China
Liyou Qiu
Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural University, Zhengzhou, P.R. China
Yuncan Ai
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China

Краткое описание

Ethylene biosynthesis and function in Agaricus bisporus (the button mushroom) remain uncertain. The enzyme activities of 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) and ACC oxidase (ACO) were detectable in A. bisporus AS2796 and inhibited by α-aminooxyacetic acid and Co2+. We cloned and sequenced 2 ACS genes (Ab-ACS1 and Ab-ACS2) and 1 ACO gene (Ab-ACO) from the mushroom strain. Ab-ACS1 and Ab-ACS2 demonstrated low amino acid sequence similarity. Ab-ACO demonstrated an amino acid sequence completely identical to that of ACO1_AGABI from A. bisporus. Antisense ACO significantly reduced ACO gene expression level, ACO enzyme activity, and ethylene production in the mushroom transformants. The transformants grew faster than the wild-type strain in sterilized compost and normally formed primordia when cultivated in sterilized compost with the sterilized casing vermiculite, but the wild-type strain did not. Our results show that ethylene is synthesized in button mushrooms via the ACC pathway. Ethylene inhibited button mushroom mycelial growth and development.


Articles with similar content:

Cloning and Characterization of Polyphenoloxidase DNA from Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.8, 2006, issue 1
Akiko Matsumoto-Akanuma, Naohito Ohno, Akihiko Yamagishi, Masuro Motoi
Purification and Characterization of a Novel Protease from the Inky Cap Mushroom, Coprinopsis atramentaria (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 4
Hexiang Wang, Wenwen Chen, Xueran Geng, Tzi Bun Ng, Weiwei Zhang
Enhanced Production of Polysaccharide Through the Overexpression of Homologous Uridine Diphosphate Glucose Pyrophosphorylase Gene in a Submerged Culture of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 5
Meng-Fei Ren, Jun-Wei Xu, Rui Liu, Huan-Jun Li, Sen-Lin Ji
Cloning and Characterization of Laccase DNA from the Royal Sun Medicinal Mushroom, Agaricus brasiliensis (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 4
Akiko Matsumoto-Akanuma, Naohito Ohno, Akihiko Yamagishi, Satoshi Akanuma, Masuro Motoi
Isolation of the Entomopathogenic Fungal Strain Cod-MK1201 from a Cicada Nymph and Assessment of Its Antibacterial Activities
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 1
Woranan Nakbanpote, Aphidech Sangdee, Kusavadee Sangdee