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Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 22, 2020 Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v17.i8.10
pages 701-712

Stimulation of the Antioxidative and Antimicrobial Potential of the Blood Red Bracket Mushroom Pycnoporus sanguineus (Higher Basidiomycetes)

Magdalena Jaszek
Department of Biochemistry, Maria Curie-Sktodowska University, Lublin, Poland
Monika Osinska-Jaroszuk
Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland
Justyna Sulej
Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland
Anna Matuszewska
Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland
Dawid Stefaniuk
Department of Biochemistry, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, Lublin, Poland
Kamil Maciag
Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland
Jolanta Polak
Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland
Lukasz Matuszewski
Pediatric Orthopedic and Rehabilitation Clinic, Medical University of Lublin, Lublin, Poland
Krzysztof Grzywnowicz
Department of Biochemistry, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, Lublin, Poland

Краткое описание

The antioxidative and antibacterial properties of low-molecular-weight secondary metabolite subfractions (ex-LMS) from cultures of Pycnoporus sanguineus cultivated under different temperature conditions (25°C [ex-LMSa] and 30°C [ex-LMSb]) were assessed. The antioxidative properties were studied using chemiluminometric measurement, an ABTS assay, and a DPPH reduction rate assay with Trolox and ascorbic acid as the control. The values noted for the ex-LMSb were significantly higher than those for ex-LMSa: 97%, 52%, and 31% for chemiluminometric measurement, the ABTS assay, and the DPPH assay, respectively, at a concentration of 50 µg/mL. Half-maximal effective concentrations reached 4.17 µg/mL for chemiluminometric measurement, 47.25 µg/mL for the ABTS assay, and 51.46 µg/mL for DPPH assay. Toxicity tests against Vibrio fischeri yielded 99.8% for ex-LMSa and 99.85% for ex-LMSb. Antibacterial activity toward Staphylococcus aureus was observed in the ex-LMSb fractions (inhibition zone, 23.5 mm; minimum inhibitory concentration, 0.12 mg/mL). Scanning electron microscopy images exhibited severe disruption of the bacterial cells treated with ex-LMSb compared with the control. The results obtained suggest that the extracellular fluid isolated from P. sanguineus−submerged cultures might be a good source of antioxidative and antibacterial compounds. In addition, the increase in the culture temperature evidently enhanced the bioactive properties of the preparation.