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International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v2.i1.70
9 pages

Basidioma Development and Genetic Recombination of Somatic Hybrids with Compatible and Incompatible Strains by Protoplast Fusion in Genera Pleurotus (Fr.) P. Karst. and Ganoderma P. Karst.

Young Bok Yoo
Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, South Korea
Beom Gi Kim
Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, South Korea
Kang Hyo Lee
Applied Microbiology Division, National Institute of Agricultural Science and Technology, RDA, Suweon 441-707, South Korea
Mi Jeong Jeong
Molecular Genetics Division, National Institute of Agricultural Science and Technology, RDA, Suweon 441 -707, Korea
Soo Chul Park
Molecular Genetics Division, National Institute of Agricultural Science and Technology, RDA, Suweon 441 -707, Korea

Краткое описание

Somatic hybrids of intercompatible and interincompatible strains were obtained by protoplast fusion. The fusion products between compatible strains, Pleurotus ostreatus and P. florida, formed heterokaryons, while fusants between incompatible strains such as P. cornucopiae + P. florida, P. ostreatus + Ganoderma applanatum, and P. florida + Ganoderma lucidum formed synkaryons that retained genes from both parents. The heterokaryons showed the same level of basidioma development. In contrast, the synkaryons showed unique characteristics including clamp connection formation at mitosis, either partner basidioma development, and abnormal segregation and recombination compared with intercompatible strains. Synkaryons can be classified into homokaryotic and heterokaryotic type.


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