Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v19.i5.80
pages 467-475

Preserving and Maintaining Culinary-Medicinal Royal Sun Mushroom, Agaricus brasiliensis (Agaricomycetes), in Sterile Distilled Water

L.M. Vargas-del-Rlo
Bioprocess Plant, Engineering Department, Universidad de Caldas, Manizales, Colombia
Sandra Montoya
Agricultural Biotechnology Institute, Universidad de Caldas, Manizales, Colombia
J.C. Sepulveda-Arias
Infection and Immunity Research Group, Health Sciences Faculty, Universidad Tecnologica de Pereira, Pereira, Colombia

Краткое описание

Strains of Agaricus brasiliensis require special procedures for conservation. Thus, the objective of this research was to establish conservation and maintenance procedures A. brasiliensis strain PSWC838 from the University of Pennsylvania (ABWC838) and an A. brasiliensis strain from the Fujian Agriculture and Forestry University (ABC). The medium in which mycelia grew the quickest for both strains was selected using a multifactorial design with 2 strains, 4 culture media, and incubation for 5 different times; the growth rate (mm/day) was the response variable. Analysis of variance showed that the potato dextrose agar (PDA) medium and potato extract did not display a significantly different growth rate, and PDA was selected for safety reasons. We also evaluated the viability of the strains grown on PDA and 0.2% activated carbon after 3 months of storage in sterile distilled water. A factorial design was applied with 2 factors, the strain and incubation for 10 different times. The Tukey post hoc test indicated that ABC showed quicker and more homogeneous growth than ABWC838. Finally, the results showed that pieces of mycelium of ABC and ABWC838 strains inoculated on the PDA medium with 0.2% activated carbon and preserved in sterile distilled water at 18 ± 1°C showed 100% viability after 3 months of storage. Moreover, the results of semiquantitative biochemical tests confirmed that the production of laccases and amylases was preserved in these strains after storage in sterile water, enhancing their ability to degrade substrates containing lignin and starchy waste.

Articles with similar content:

Inhibiting Effect of Medicinal Mushroom Lentinus edodes (Berk.) Sing. (Agaricomycetideae) on Aflatoxin Production by Aspergillus parasiticus Speare
International Journal of Medicinal Mushrooms, Vol.2, 2000, issue 3
Anna Adele Fabbri, Slaven Zjalic, Alessandra Ricelli, Enrico Finotti, Massimo Reverberi, Valeria Tasca, Corrado Fanelli
Cultivation of Lentinus squarrosulus (Mont.) Singer on Sawdust of Selected Tropical Tree Species
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Emmanuel Oluwadare Akpaja, Abbot Oghenekaro, John Aroye Okhuoya
Cultivation of Medicinal Mushrooms on Spruce Sawdust Fermented with a Liquid Digestate from Biogas Stations
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 3
Ivan Jablonský, Adam Brezáni, Katerina Svobodova, Pavel Tlustoš
Production of Ligninolytic Enzymes by Oyster Mushroom Pleurotus ostreatus (Jacq.:Fr.) P. Kumm. Under Different Nutritional Conditions
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Nona A. Mikiashvili
Studies on Laccase and Biomass Production In Vitro and Culture by a Mexican Wild Strain of Agaricus bisporus (J.Lge) Imbach: a Comparison with Commercial Strains
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
A. Rodriguez-Estrada, Gerardo Mata