Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v19.i1.20
pages 17-25

In Vitro and In Vivo Antidiabetic Evaluation of Selected Culinary-Medicinal Mushrooms (Agaricomycetes)

Varinder Singh
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, Punjab, India
Gurleen Kaur Bedi
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, Punjab, India
Richa Shri
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Punjab, India

Краткое описание

Management of type 2 diabetes by delaying or preventing glucose absorption using natural products is gaining significant attention. Edible mushrooms are well documented for their nutritional and medicinal properties. This investigation was designed to evaluate the antidiabetic activity of aqueous extracts of selected culinary-medicinal mushrooms, namely, Pleurotus ostreatus, Calocybe indica, and Volvariella volvacea, using in vitro models (α-amylase inhibition assay, glucose uptake by yeast cells, and glucose adsorption capacity). The most active extract was subsequently examined in vivo using the oral starch tolerance test in mice. All prepared extracts showed dose-dependent inhibition of α-amylase and an increase in glucose transport across yeast cells. C. indica extract was the most active α-amylase inhibitor (half-maximal inhibitory concentration, 18.07 ± 0.75 mg/mL) and exhibited maximum glucose uptake by yeast cells (77.53 ± 0.97% at 35 mg/mL). All extracts demonstrated weak glucose adsorption ability. The positive in vitro tests for C. indica paved the way for in vivo studies. C. indica extract (200 and 400 mg/kg) significantly (P < 0.05) reduced postprandial blood glucose peaks in mice challenged with starch. The extract (400 mg/kg) and acarbose normalized blood glucose levels at 180 minutes, when they were statistically similar to values in normal mice. Thus, it may be concluded that the antidiabetic effect of C. indica is mediated by inhibition of starch metabolism (α-amylase inhibition), increased glucose uptake by peripheral cells (promotion of glucose uptake by yeast cells), and mild entrapment (adsorption) of glucose. Hence, C. indica can be developed as antidiabetic drug after detailed pharmacological studies.


Articles with similar content:

Oral Hypoglycemic Activity of Culinary-Medicinal Mushrooms Pleurotus ostreatus and P. cystidiosus (Higher Basidiomycetes) in Normal and Alloxan-Induced Diabetic Wistar Rats
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 4
C.A. Wanigatunga, W.J.A.B.N. Jayasuriya, T.S. Suresh, G.H. Fernando, D. Thusitha U. Abeytunga
Assessment of Anti-inflammatory, Lipid Peroxidation and Acute Toxicity of Extracts Obtained From Wild Higher Basidiomycetes Mushrooms Collected From Akure (Southwest Nigeria)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 6
Antonio Nieto-Camacho, Victor O. Oyetayo, Baldomero Esquivel Rodriguez, Manuel Jimenez
Antioxidant and Enzyme Inhibitory Activities of Extracts from Wild Mushroom Species from Turkey
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 4
Abdurrahman Aktumsek, Gokhan Zengin, Mehmet Sefa Kocak, Mehmet Cemil Uren, Marcello Locatelli, Halil Gungor, Cengiz Sarikurkcu
Pharmacological Studies on Certain Mushrooms from China
International Journal of Medicinal Mushrooms, Vol.3, 2001, issue 4
Vincent Eng Choo Ooi
Hypoglycemic Effects of Mycelia Produced from a Submerged Culture of Phellinus linteus (Berk. et Curt.) Teng (Aphyllophoromycetideae) in Streptozotocin-lnduced Diabetic Rats
International Journal of Medicinal Mushrooms, Vol.3, 2001, issue 1
Dong-Hyun Kim, Young-Sun Choi, Nam-Jung Hur, Chi-Hyun Song, Jong-Won Yun, Surajit Das, Byung-Kun Yang