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International Journal on Algae
SJR: 0.219 SNIP: 0.261 CiteScore™: 0.24

ISSN Печать: 1521-9429
ISSN Онлайн: 1940-4328

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International Journal on Algae

DOI: 10.1615/InterJAlgae.v2.i1.30
pages 22-30

Variability of intracellular elemental concentrations in freshwater phytoplankton estimated using SEM XRMA in relation to the conditions of analysis

V. Krivtsov
The University of Manchester, School of Biological Sciences, 1.604 Stopford Building, Oxford Road, Manchester M13 9PT, UK
E. Bellinger
Dept. of Environmental Sciences and Policy, Central European University, Nador utca 9, 1051 Budapest, Hungary
D. Sigee
School of Biological Sciences, University of Manchester, 1.8000 Stopford Building, Oxford Road, Manchester M13 9PT, UK

Краткое описание

Variability of elemental concentrations in cells of Ceratium hirundinella and Anabaena sp. measured by SEM XRMA were studied in relation to the conditions of the analyses. A point mode of analyses proved to be very variable due to the high heterogeneity of bioobjects, while results obtained by a medium-size reduced raster gave the best estimate of the concentrations in question. Larger sizes of reduced raster led to an underestimation of the measured intracellular concentrations probably due to a relatively high share of a cell wall and picking up void space, while results gained with smaller sizes of a reduced raster were subject to a high variability characteristic of a point mode analysis. Application of a medium-sized reduced raster could be recommended for measurements of intracellular elemental concentrations of freshwater phytoplankton species.