RT Journal Article
ID 04a4a9a4319cda42
A1 Zhang, Hua
A1 Gong, Feng 
A1 Feng, Yongjun
A1 Zhang, Changkai
T1 Flammulin Purified from the Fruit Bodies of <i>Flammulina velutipes</i> (Curt.:Fr.) P.Karst
JF International Journal of Medicinal Mushrooms
JO IJM
YR 1999
FD 1999-03-01
VO 1
IS 1
SP 89
OP 92
K1 Antitumor
K1 crystallization
K1 extract
K1 flammulin
K1 <i>Flammulina velutipes</i> 088.
AB Flammulin, an antitumor substance, was purified from the aqueous extract of basidiomes of <i>Flammulina velutipes</i> (Curt.: Fr.) P.Karst. It was judged homogenous by SDS-polyacrylamide gel electrophoresis. The purification procedure included ammonium sulfate precipitation, ethanol fractionation, DEAE-Sephadex anion-exchange chromatography, crystallization, and recrystallization. Flammulin was crystallized with microdialysis against a polyethylene glycol sodium phosphate buffer. The crystals were rhombohedron-shaped and easy to melt when the crystallization conditions were changed. With a molecular mass of 23,700, flammulin was characterized by its lack of Met. Amino acid composition analysis showed that the numbers of acidic amino acids are more than that of basic amino acids, but the isoelectric point detected by analytical isoelectric focusing was at pH8.9.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,6f35eacf6176a1b5,04a4a9a4319cda42.html