RT Journal Article
ID 2e0fc7532bb25b1b
A1 Radakovic, Milena
A1 Djelic, Ninoslav
A1 Stevanovic, Jevrosima
A1 Sokovic, Marina
A1 Radovic, Dejan
A1 Van Griensven, Leonardus Johannes Lambertus Donatus
A1 Stanimirovic, Zoran
T1 Evaluation of the Antigenotoxic Effects of the Royal Sun Mushroom, Agaricus brasiliensis (Higher Basidiomycetes) in Human Lymphocytes Treated with Thymol in the Comet Assay
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2015
FD 2015-05-08
VO 17
IS 4
SP 321
OP 330
K1 medicinal mushrooms
K1 thymol
K1 Agaricus brasiliensis
K1 quercetin
K1 human lymphocytes
K1 comet assay
AB The aim of this investigation was to evaluate the possible protective activity of Agaricus brasiliensis (=A. blazei sensu Murrill) ethanol extract against thymol-induced DNA damage in human lymphocytes. Before we studied the possible interaction of thymol and A. brasiliensis extract, each component was tested in the comet assay. Thymol significantly increased DNA damage in human lymphocytes at higher concentrations (20, 50, 100, 150, and 200 µg/mL), whereas no genotoxic effect of A. brasiliensis ethanol extract was observed. In simultaneous treatment with thymol (200 µg/mL) and A. brasiliensis ethanol extract (50, 100, 150, and 200 µg/mL), the latter failed to reduce a thymol-induced DNA damaging effect regardless of the applied concentrations. To confirm that thymol induces DNA damage via reactive oxygen species, we performed cotreatment with quercetin. Cotreatment with quercetin (100 and 500 µmol/L) significantly reduced DNA damage caused by thymol (200 µg/mL), indicating that thymol exhibits genotoxicity mainly through induction of reactive oxygen species.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,5b711a741ddf87a8,2e0fc7532bb25b1b.html