RT Journal Article ID 2e0fc7532bb25b1b A1 Radakovic, Milena A1 Djelic, Ninoslav A1 Stevanovic, Jevrosima A1 Sokovic, Marina A1 Radovic, Dejan A1 Van Griensven, Leonardus Johannes Lambertus Donatus A1 Stanimirovic, Zoran T1 Evaluation of the Antigenotoxic Effects of the Royal Sun Mushroom, Agaricus brasiliensis (Higher Basidiomycetes) in Human Lymphocytes Treated with Thymol in the Comet Assay JF International Journal of Medicinal Mushrooms JO IJM YR 2015 FD 2015-05-08 VO 17 IS 4 SP 321 OP 330 K1 medicinal mushrooms K1 thymol K1 Agaricus brasiliensis K1 quercetin K1 human lymphocytes K1 comet assay AB The aim of this investigation was to evaluate the possible protective activity of Agaricus brasiliensis (=A. blazei sensu Murrill) ethanol extract against thymol-induced DNA damage in human lymphocytes. Before we studied the possible interaction of thymol and A. brasiliensis extract, each component was tested in the comet assay. Thymol significantly increased DNA damage in human lymphocytes at higher concentrations (20, 50, 100, 150, and 200 µg/mL), whereas no genotoxic effect of A. brasiliensis ethanol extract was observed. In simultaneous treatment with thymol (200 µg/mL) and A. brasiliensis ethanol extract (50, 100, 150, and 200 µg/mL), the latter failed to reduce a thymol-induced DNA damaging effect regardless of the applied concentrations. To confirm that thymol induces DNA damage via reactive oxygen species, we performed cotreatment with quercetin. Cotreatment with quercetin (100 and 500 µmol/L) significantly reduced DNA damage caused by thymol (200 µg/mL), indicating that thymol exhibits genotoxicity mainly through induction of reactive oxygen species. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,5b711a741ddf87a8,2e0fc7532bb25b1b.html