RT Journal Article
ID 2f90ae8735008829
A1 Kachlishvili, Eva
A1 Kobakhidze, Aza
A1 Rusitashvili, Mariam
A1 Tsokilauri, Ana
A1 Elisashvili, Vladimir I.
T1 Comparison of Mono- and Dikaryotic Medicinal Mushrooms Lignocellulolytic Enzyme Activity
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2019
FD 2019-12-11
VO 21
IS 11
SP 1115
OP 1122
K1 mono- and dikaryotic basidiomycetes
K1 submerged cultivation
K1 cellulase
K1 laccase
K1 manganese peroxidase
K1 medicinal mushrooms
AB Mono- and dikaryotic medicinal mushroom strains isolated from four wood-rotting basidiomycete fruiting bodies were comparatively evaluated for laccase, manganese peroxidase, cellulase, and xylanase activities in submerged cultivation in glucose or mandarin peel−containing media. Mandarin peels appeared to be the preferred growth substrate for laccase production by both mono- and dikaryotic Trametes multicolor 511 and T. versicolor 5 while glucose favored laccase activity secretion by Pleurotus ostreatus 2175. Lignocellulose-deconstructing enzyme profiles were highly variable between the studied monokaryotic and dikaryotic strains. A distinctive superiority of enzyme activity of the dikaryotic Trametes versicolor 5 and P. ostreatus 2175 over the same species monokaryotic isolates was revealed. By contrast, laccase, cellulase, and xylanase activities of the monokaryotic strain of T. multicolor 511 were rather higher than those in the dikaryotic culture. At the same time, hydrolases activity of Schizophyllum commune 632 was practically independent of the origin of the fungal culture. The results suggest that the monokaryotic isolates derived from the basidiomycetes fruiting bodies inherit parental properties but the capacity of individual monokaryotic cultures to produce lignocellulose-deconstructing enzymes can vary considerably.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,25f9c61f6dbb0698,2f90ae8735008829.html