RT Journal Article ID 2f90ae8735008829 A1 Kachlishvili, Eva A1 Kobakhidze, Aza A1 Rusitashvili, Mariam A1 Tsokilauri, Ana A1 Elisashvili, Vladimir I. T1 Comparison of Mono- and Dikaryotic Medicinal Mushrooms Lignocellulolytic Enzyme Activity JF International Journal of Medicinal Mushrooms JO IJM YR 2019 FD 2019-12-11 VO 21 IS 11 SP 1115 OP 1122 K1 mono- and dikaryotic basidiomycetes K1 submerged cultivation K1 cellulase K1 laccase K1 manganese peroxidase K1 medicinal mushrooms AB Mono- and dikaryotic medicinal mushroom strains isolated from four wood-rotting basidiomycete fruiting bodies were comparatively evaluated for laccase, manganese peroxidase, cellulase, and xylanase activities in submerged cultivation in glucose or mandarin peel−containing media. Mandarin peels appeared to be the preferred growth substrate for laccase production by both mono- and dikaryotic Trametes multicolor 511 and T. versicolor 5 while glucose favored laccase activity secretion by Pleurotus ostreatus 2175. Lignocellulose-deconstructing enzyme profiles were highly variable between the studied monokaryotic and dikaryotic strains. A distinctive superiority of enzyme activity of the dikaryotic Trametes versicolor 5 and P. ostreatus 2175 over the same species monokaryotic isolates was revealed. By contrast, laccase, cellulase, and xylanase activities of the monokaryotic strain of T. multicolor 511 were rather higher than those in the dikaryotic culture. At the same time, hydrolases activity of Schizophyllum commune 632 was practically independent of the origin of the fungal culture. The results suggest that the monokaryotic isolates derived from the basidiomycetes fruiting bodies inherit parental properties but the capacity of individual monokaryotic cultures to produce lignocellulose-deconstructing enzymes can vary considerably. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,25f9c61f6dbb0698,2f90ae8735008829.html