RT Journal Article
ID 3818d4963fba2ee9
A1 Cateni, Francesca
A1 Zacchigna, Marina
A1 Bavisotto, Celeste Caruso
A1 Procida, Giuseppe
A1 Bonaventura, Giuseppe
A1 Saporita, Paola
A1 Calvo, Roberta
A1 Venturella, Giuseppe
A1 Gargano, Maria Letizia
T1 Structural Characterization of Polysaccharides of a Productive Strain of the Culinary-Medicinal King Oyster Mushroom, Pleurotus eryngii (Agaricomycetes), from Italy
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-08-17
VO 20
IS 8
SP 717
OP 726
K1 Pleurotus eryngii
K1 polysaccharides
K1 antioxidant activity
K1 MTT assay
K1 medicinal mushrooms
AB A preliminary biological investigation of the dry basidiomata of strain C-142-c of Pleurotus eryngii has shown significant antioxidant activity. Two different polysaccharides (PEPS-A1 and PEPS-A2) were isolated from the cultivated edible mushroom, P. eryngii C-142-c strain. Based on acid hydrolysis, methylation analysis, and nuclear magnetic resonance experiments (1H, 13C, distortionless enhancement by polarization transfer, double quantum filtered correlation spectroscopy, total correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single-quantum correlation spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structures of the repeating unit of PEPS-A1 and PEPS-A2 were established as follows: (l)PEPS-Al (α-glucan): [→6)-α-D-Glcp-(1→6)-α-D-Glcp-(l→]n; and (2) PEPS-A2 (β-glucan): [→6)-β-D-Glcp-(1→6)-β-D-Glcp-(l→]n. The antioxidant activity of PEPS-A1 and PEPS-A2 was evaluated as hydroxyl radical scavenging activity. PEPS-A1 and PEPS-A2 showed SC50 values of 400 μg/mL and 122 μg/mL, respectively, suggesting their possible use as a dietary supplement in functional foods. The polysaccharides were tested for their activity on cell viability using a colorectal adenocarcinoma cell line (HT-29). Both polysaccharides affected cell viability after 48 and 72 hours of treatment, inducing the death of 50% of HT-29 cells between 0.25 and 1 μg/mL and between 0.5 and 1 μg/mL, respectively, for PEPS-A1 and PEPS-A2. These results are promising for future applications of these mushroom-derived polysaccharides as antioxidants and antitumor agents.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,66746bdc1bb430f2,3818d4963fba2ee9.html