RT Journal Article
ID 599c6cc54a7e61e7
A1 Chun, Kyung-Soo
A1 Kang, Jee-Young
A1 Kim, Ok Hee
A1 Kang, Hoil
A1 Surh, Young-Joon
T1 Effects of Yakuchinone A and Yakuchinone В on the Phorbol Ester-Induced Expression of COX-2 and iNOS and Activation of NF-kB in Mouse Skin
JF Journal of Environmental Pathology, Toxicology and Oncology
JO JEP(T)
YR 2002
FD 2002-06-01
VO 21
IS 2
OP 9
AB Certain medicinal plants contain anti-inflammatory and antioxidative substances that can exert chemopreventive effects. Our previous studies have demonstrated that the methanol extract of Alpinia oxyphylla Miquel (Zingiberaceae) inhibits tumor promotion in mouse skin. Two major diarylheptanoids named yakuchinone A (l-[4'-hydroxy-3'-methoxyphenyl]-7-phenyl-3-heptanone) and yakuchinone В (l-[4'-hydroxy-3'-methoxyphenyl]-7-phenylhept-l-en-3-one) have been isolated from this medicinal plant. Both compounds have strong inhibitory effects on the synthesis of prostaglandins and leukotrienes in vitro. In the present work, we show that both yakuchinone A and yakuchinone В inhibit the expression of cyclooxygenase-2 (COX-2) and of inducible nitric oxide synthase (iNOS) as well as the expression of tumor necrosis factor (TNF)-a mRNA in mouse skin treated with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Topical application on mouse skin of these diarylheptanoids also attenuated the TPA-induced DNA binding activity of the ubiquitous eukaryotic transcription factor NF-kB that plays a crucial role in regulating the expression of the aforementioned proinflammatory enzymes and cytokines in response to a wide variety of external stimuli. These findings suggest that diarylheptanoids contained in Alpinia oxyphylla down-regulate COX-2 and iNOS expression through suppression of NF-kB activation in the TPA-treated mouse skin.
PB Begell House
LK https://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,4ac6c8531cba5202,599c6cc54a7e61e7.html