RT Journal Article ID 5ddc9a6e6858a734 A1 Ali, Sharjahan Mohamed A1 Raman, Jegadeesh A1 Lakshmanan, Hariprasath A1 Ling, Tau Chuan A1 Phan, Chia Wei A1 Tan, Yee Shin A1 Sabaratnam, Vikineswary T1 Surface Decoration of Selenium Nanoparticles by Proteins from the Culinary-Medicinal Shiitake Mushroom, Lentinus edodes (Agaricomycetes), for Enhanced Fibrinolytic Activity JF International Journal of Medicinal Mushrooms JO IJM YR 2018 FD 2018-11-02 VO 20 IS 11 SP 1021 OP 1030 K1 fibrinolytic activity K1 Lentinus edodes K1 medicinal mushrooms K1 nanoparticles K1 selenium K1 shiitake AB Lentinus edodes (shiitake mushroom) has exhibited fibrinolytic activity. We synthesized and characterized selenium nanoparticles (SeNPs) using protein precipitated from the mushroom. We also investigated the fibrinolytic activity of the SeNPs. The proteins from a crude extract of L. edodes were recovered through the use of aqueous 2-phase separation, and these we used as the capping agent in SeNP biosynthesis. We characterized the SeNPs using UV-visible spectrophotometry, field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX), transmission electron microscopy (TEM), particle size distribution analysis, and Fourier transform infrared spectroscopy (FT-IR). The fibrinolytic capability of the SeNPs was tested through an in vitro fibrin plate assay. The UV-visible spectra showed maximal absorbance at 220 nm. FESEM images showed that the SeNPs were dispersed and did not clump. The TEM images revealed a spherical shape and average size of the SeNPs. The particle size distribution analysis confirmed the mean size of the SeNPs at 64.53 nm. A strong signal for the presence of selenium was observed in the EDX analysis. The FT-IR spectrum revealed the involvement of protein functional groups in the reduction of sel-enite. Overall, the SeNPs capped with protein from shiitake mushroom were effective as an in vitro fibrinolytic agent. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,6e2de2a705b6ffd3,5ddc9a6e6858a734.html