RT Journal Article
ID 5ddc9a6e6858a734
A1 Ali, Sharjahan Mohamed
A1 Raman, Jegadeesh
A1 Lakshmanan, Hariprasath
A1 Ling, Tau Chuan
A1 Phan, Chia Wei
A1 Tan, Yee Shin
A1 Sabaratnam, Vikineswary
T1 Surface Decoration of Selenium Nanoparticles by Proteins from the Culinary-Medicinal Shiitake Mushroom, Lentinus edodes (Agaricomycetes), for Enhanced Fibrinolytic Activity
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-11-02
VO 20
IS 11
SP 1021
OP 1030
K1 fibrinolytic activity
K1 Lentinus edodes
K1 medicinal mushrooms
K1 nanoparticles
K1 selenium
K1 shiitake
AB Lentinus edodes (shiitake mushroom) has exhibited fibrinolytic activity. We synthesized and characterized selenium nanoparticles (SeNPs) using protein precipitated from the mushroom. We also investigated the fibrinolytic activity of the SeNPs. The proteins from a crude extract of L. edodes were recovered through the use of aqueous 2-phase separation, and these we used as the capping agent in SeNP biosynthesis. We characterized the SeNPs using UV-visible spectrophotometry, field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX), transmission electron microscopy (TEM), particle size distribution analysis, and Fourier transform infrared spectroscopy (FT-IR). The fibrinolytic capability of the SeNPs was tested through an in vitro fibrin plate assay. The UV-visible spectra showed maximal absorbance at 220 nm. FESEM images showed that the SeNPs were dispersed and did not clump. The TEM images revealed a spherical shape and average size of the SeNPs. The particle size distribution analysis confirmed the mean size of the SeNPs at 64.53 nm. A strong signal for the presence of selenium was observed in the EDX analysis. The FT-IR spectrum revealed the involvement of protein functional groups in the reduction of sel-enite. Overall, the SeNPs capped with protein from shiitake mushroom were effective as an in vitro fibrinolytic agent.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,6e2de2a705b6ffd3,5ddc9a6e6858a734.html