RT Journal Article
ID 60eb5ad86bf252ad
A1 Shah, Muhammad Dawood
A1 Gnanaraj, Charles
A1 Khan, Mohammad Shaheen
A1 Iqbal, Mohammad
T1 Dillenia suffruticosa L. Impedes Carbon Tetrachloride−Induced Hepatic Damage by Modulating Oxidative Stress and Inflammatory Markers in Rats
JF Journal of Environmental Pathology, Toxicology and Oncology
JO JEP(T)
YR 2015
FD 2015-02-27
VO 34
IS 2
SP 133
OP 152
K1 ultrastructure
K1 antioxidant
K1 immunohistochemistry
K1 Dillenia suffruticosa
K1 oxidative stress
K1 inflammatory markers
AB Dillenia suffruticosa L. (Dilleniaceae) is used in traditional medicine for protection against various
diseases. The current study was designed to investigate the bioactive compounds and hepatoprotective potential
of methanol leaves extract of D. suffruticosa against carbon tetrachloride (CCl4)-induced hepatic oxidative injury.
Sprague Dawley rats were pretreated with methanol extract of D. suffruticosa leaves (200, 300, and 400 mg/kg
body weight [bwt]) once daily for 14 days followed by two doses of CCl4 (1.0 mL/kg bwt). After 2 weeks the rats
were sacrificed and hepatoprotective analysis was performed. The identified bioactive compounds include phenol
(1.39%); benzyl alcohol (2.04%); 2H-pyran-2-one, 4,6-dimethyl (1.19%); phenol, 2,4-bis(1,1-dimethylethyl)
(0.83%); dodecanoic acid (0.84%); hexadecanoic acid, methyl ester (2.66%); n-hexadecanoic acid (0.96%); and
phytol (2.13%). The administration of D. suffruticosa significantly depleted the elevation of enzymatic levels of
alanine transaminase and aspartate transaminase (4% to 59% recovery), reduced the extent of malondialdehyde
production (13% to 79% recovery), elevated the level of reduced glutathione (5% to 21% recovery), and increased
the activities of antioxidant enzymes (0.43% to 35% recovery). Histopathological analyses by light and electron
microscopy revealed that the plant extract protects the liver from the toxic effects of CCl4 and cured lesions such
as necrosis and fatty degeneration. It also decreased hepatocyte injuries such as irregular lamellar organization and
dilations in endoplasmic reticulum. Immunohistochemical studies indicate the formation of 8-hydroxy-2'-deoxyguanosine
(8-OHdG) and 4-hydroxyl-2-nonenal (HNE)-modified protein adducts. In addition, the overexpression of
the inflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and prostaglandin E2 (PGE2)
are reduced. Hence, we find D. suffruticosa to be a good source of bioactive compounds with hepatoprotective effects
against CCl4-induced oxidative damage.
PB Begell House
LK https://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,0972237e0d0d4968,60eb5ad86bf252ad.html