%0 Journal Article %A Veselovsky, Mycola S. %A Shipshina, Maria S. %D 2011 %I Begell House %K Dorsal root ganglion, dorsal horn spinal cord, co-culture, synaptic transmission, NMDA-receptors, non-NMDA-receptors, GABA, glycine, evoked postsynaptic currents %N 1 %P 1-13 %R 10.1615/IntJPhysPathophys.v2.i1.10 %T Characteristic of Sensory Neurotransmission in the Co-Culture of Both the Rat Dorsal Root Ganglion and the Rat Dorsal Horn Spinal Cord Neurons %U https://www.dl.begellhouse.com/journals/6ec4ba27650016b1,4233e80d10b9a4d1,6f125d476fa00fdf.html %V 2 %X We examined properties of chemical neurotransmission at the level of primary afferent inputs into spinal cord with the new easy-to-use in vitro model of contiguously-cultivated dissociated both the dorsal root ganglion neurons (DRG) and the dorsal horn spinal cord neurons (DHSC) from newborn rats. The results of our studies showed presence of excitatory and inhibitory DRG neurons synapses on the cells of DHSC. The excitatory afferent signaling in such synapses was mediated by presynaptic release of glutamate and a following activation of both NMDA- and non-NMDA-receptor subclasses. In these cases the activation of non-NMDA- receptors makes a main contribution to realization excitatory postsynaptic effects. Either glycine- or GABA-ergic DRG neurons were involved in transmission of inhibitory signals to the DHSC neurons. However, in vast majority of examined neuronal pairs the inhibitory synaptic transmission was mediated by presynaptic release of glycine. As distinct from previous similar methods proposed here in vitro model of co-culture both the DRG and the DHSC neurons allows using a modern technical approaches to examination of the transmission of somatosensory information from the periphery to the CNS to the best advantage. The described model could be acceptable for detailed studding of specific properties of primary afferent synapses. %8 2011-03-01