%0 Journal Article %A Yang, Hairui %A Sun, Wenbo %A Zhang, Jinsong %A Zhang, Yaping %A Yang, Yan %A Wu, Di %A Liu, Yanfang %A Qiankun, Zheng %A Min, Li %A Wang, Wenhan %A Jia, Wei %D 2018 %I Begell House %K apoptosis, autophagy, By-1, cell proliferation, medicinal mushrooms, Taiwanofungus camphoratus %N 4 %P 321-335 %R 10.1615/IntJMedMushrooms.2018025836 %T Autophagy Inhibition Enhances SPCA-1 Cell Proliferation Inhibition Induced by By-1 from the Stout Camphor Medicinal Mushroom, Taiwanofungus camphoratus (Agaricomycetes) %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,31a39db86881b79c,7895d53d647838f8.html %V 20 %X Taiwanofungus camphoratus has been reported to have antitumor effects against various cancer cells. The aim of this study was to investigate the direct inhibitory effect of By-1 (3-isobutyl-l-methoxy-4-[4'-(3-methylbut-2-enyloxy)phenyl]-1H-pyrrole-2,5-dione), a compound from spent broth from submerged cultures of T. camphoratus, on human lung adenocarcinoma cells and to determine the molecular mechanism underlying this effect. The growth-inhibitory assay and colony formation assay showed that cell viability was significantly decreased. A By-1 concentration of 300 μmol/L caused 73.55% cell death and at a concentration of 240 μmol/L led to a 58% reduction in the number of colonies. The wound-healing assay showed that the distance of migration was 0.3 times shorter than that of untreated cells. Flow cytometry revealed that By-1 could suppress DNA synthesis, cause cell cycle arrest at the S phase, and induce apoptosis in a reactive oxygen species-dependent manner. Furthermore, the expression of caspase-3 and P53 was 4 times higher than that in untreated cells, and the antiapoptotic protein Bcl-2 was decreased 2 times compared with the protein in untreated cells. It is interesting to note that apoptosis and autophagy were both induced during treatment with By-1, and autophagy inhibition decreased cell proliferation. By-1 potently inhibited the growth of SPCA-1 cells by inducing cell cycle arrest and apoptosis. The combination of proapoptosis agents and antiautophagy agents could effectively enhance anticancer efficacy, which may be a new strategy in treating non-small cell lung cancer. %8 2018-05-16