%0 Journal Article
%A Yang, Hairui
%A Sun, Wenbo
%A Zhang, Jinsong
%A Zhang, Yaping
%A Yang, Yan
%A Wu, Di
%A Liu, Yanfang
%A Qiankun, Zheng
%A Min, Li
%A Wang, Wenhan
%A Jia, Wei
%D 2018
%I Begell House
%K apoptosis, autophagy, By-1, cell proliferation, medicinal mushrooms, Taiwanofungus camphoratus
%N 4
%P 321-335
%R 10.1615/IntJMedMushrooms.2018025836
%T Autophagy Inhibition Enhances SPCA-1 Cell Proliferation Inhibition Induced by By-1 from the Stout Camphor Medicinal Mushroom, Taiwanofungus camphoratus (Agaricomycetes)
%U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,31a39db86881b79c,7895d53d647838f8.html
%V 20
%X Taiwanofungus camphoratus has been reported to have antitumor effects against various cancer cells. The aim of this study was to investigate the direct inhibitory effect of By-1 (3-isobutyl-l-methoxy-4-[4'-(3-methylbut-2-enyloxy)phenyl]-1H-pyrrole-2,5-dione), a compound from spent broth from submerged cultures of T. camphoratus, on human lung adenocarcinoma cells and to determine the molecular mechanism underlying this effect. The growth-inhibitory assay and colony formation assay showed that cell viability was significantly decreased. A By-1 concentration of 300 μmol/L caused 73.55% cell death and at a concentration of 240 μmol/L led to a 58% reduction in the number of colonies. The wound-healing assay showed that the distance of migration was 0.3 times shorter than that of untreated cells. Flow cytometry revealed that By-1 could suppress DNA synthesis, cause cell cycle arrest at the S phase, and induce apoptosis in a reactive oxygen species-dependent manner. Furthermore, the expression of caspase-3 and P53 was 4 times higher than that in untreated cells, and the antiapoptotic protein Bcl-2 was decreased 2 times compared with the protein in untreated cells. It is interesting to note that apoptosis and autophagy were both induced during treatment with By-1, and autophagy inhibition decreased cell proliferation. By-1 potently inhibited the growth of SPCA-1 cells by inducing cell cycle arrest and apoptosis. The combination of proapoptosis agents and antiautophagy agents could effectively enhance anticancer efficacy, which may be a new strategy in treating non-small cell lung cancer.
%8 2018-05-16