Доступ предоставлен для: Guest
Главная ICHMT DL Текущий год Архив Исполнительный Комитет ICHMT

MULTIPHYSICS SIMULATION OF MICROFLUIDIC REACTOR FOR POLYMERASE CHAIN REACTION

DOI: 10.1615/ICHMT.2014.IntSympConvHeatMassTransf.420
pages 565-574

Barbaros Cetin
IhsanDogramacıBilkent University, Mech. Eng. Dept. 06800 Ankara, Turkey

Ilbey Karakurt
Ihsan Dogramaci Bilkent University, Mechanical Engineering Department Microfluidics and Lab-on-a-chip Research Group 06800 Ankara, Turkey

Аннотация

Polymerase-chain-reaction (PCR) is a thermal cycling process (repeated heating and cooling of PCR solution) for amplifying DNA. PCR devices have many biomedical applications. One of the most important aspects for the success of PCR is to control the temperature of the solution precisely at the desired temperature levels required for PCR in a cyclic manner. Microfluidics offers a great advantage over conventional techniques since very small amounts of PCR solution is needed for the process to occur at the desired temperature levels. In this study, a multiphysics-based computational model is developed to assess the thermal performance of a microfluidics platform for continuous-flow PCR. The microfluidic platform consists of a spiral channel on a glass wafer with integrated chromium microheaters. The computational model couples the convection heat transfer and fluid flow within the microchannles and the electric field generated at the microheaters using COMSOL® Multiphysics software. With the current computational model, the effects of design parameter on the performance of PCR cycle can be understood and utilized for the optimization of a microfluidic PCR device. Moreover, the computational model can also be implemented for a general design tool for the design of efficient microfluidics based thermal reactors which can extend the boundaries of microfluidics technologies in biomedical and bioengineering fields.

ICHMT Digital Library

Bow shocks on a jet-like solid body shape. Thermal Sciences 2004, 2004. Pulsed, supersonic fuel jets - their characteristics and potential for improved diesel engine injection. PULSED, SUPERSONIC FUEL JETS - THEIR CHARACTERISTICS AND POTENTIAL FOR IMPROVED DIESEL ENGINE INJECTION
View of engine compartment components (left). Plots of temperature distributions in centreplane, forward of engine (right). CHT-04 - Advances in Computational Heat Transfer III, 2004. Devel... DEVELOPMENT AND CURRENT STATUS OF INDUSTRIAL THERMOFLUIDS CFD ANALYSIS
Pratt & Whitney's F-135 Joint Strike Fighter Engine under test in Florida is a 3600F class jet engine. TURBINE-09, 2009. Turbine airfoil leading edge stagnation aerodynamics and heat transfe... TURBINE AIRFOIL LEADING EDGE STAGNATION AERODYNAMICS AND HEAT TRANSFER - A REVIEW
Refractive index reconstructed field. (a) Second iteration. (b) Fourth iteration. Radiative Transfer - VI, 2010. Theoretical development for refractive index reconstruction from a radiative ... THEORETICAL DEVELOPMENT FOR REFRACTIVE INDEX RECONSTRUCTION FROM A RADIATIVE TRANSFER EQUATION-BASED ALGORITHM
Two inclusion test, four collimated sources. Radiative Transfer - VI, 2010. New developments in frequency domain optical tomography. Part II. Application with a L-BFGS associated to an inexa... NEW DEVELOPMENTS IN FREQUENCY DOMAIN OPTICAL TOMOGRAPHY. PART II. APPLICATION WITH A L-BFGS ASSOCIATED TO AN INEXACT LINE SEARCH