Transdermal Delivery of Adenosine and Eosin Y Using Microplasma Combined with FeSO<sub>4</sub> and DMSO Iontophoresis

Jaroslav Kristof; Fariha  Mustafa; Ahmad Guji  Yahaya; Marius  Blajan; Kazuo Shimizu

doi:10.1615/PlasmaMed.2021040968

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总编辑: Satoshi Hamaguchi (open in a new tab)

副主编: Gregory Fridman (open in a new tab)

Executive Editors: Klaus-Dieter Weltmann (open in a new tab) Alexander A. Fridman (open in a new tab)

每年出版 4 期

ISSN 打印: 1947-5764

ISSN 在线: 1947-5772

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Transdermal Delivery of Adenosine and Eosin Y Using Microplasma Combined with FeSO₄ and DMSO Iontophoresis

卷 11, 册 4, 2021, pp. 1-14

DOI: 10.1615/PlasmaMed.2021040968

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Jaroslav Kristof
Organization for Innovation and Social Collaboration, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu-shi, Shizuoka 432-8561, Japan

Fariha Mustafa
Graduate School of Integrated Science and Technology, Shizuoka University, Japan

Ahmad Guji Yahaya
Graduate School of Science and Technology, Shizuoka University, Japan

Marius Blajan
Organization for Innovation and Social Collaboration, Shizuoka University, Japan

Kazuo Shimizu
Department of Optoelectronics and Nanostructure Science, Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu-shi, Shizuoka 432-8011, Japan; Organization for Innovation and Social Collaboration, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu-shi, Shizuoka 432-8561, Japan; Graduate School of Medical Photonics, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu-shi, Shizuoka 432-8011, Japan; Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu-shi, Shizuoka 432-8561, Japan

摘要

One of the main roles of transdermal drug delivery is to overcome stratum cor-neum; the first barrier of the skin. We combined several types of treatments to investigate their effect on skin permeability and drug absorption. Franz diffusion cell was used to investigate the permeation and retention of adenosine through Yucatan micropig's skin. The amount of the penetrated drug and the drug retained inside the skin was measured by HPLC. We compared adenosine permeation through the untreated skin, iontophoretically pre-treated skin with FeSO₄·7H₂O in DMSO followed by microplasma treatment, iontophoretically pre-treated skin with FeSO₄·7H₂O in water followed by microplasma treatment, and treated skin with DMSO. Eosin Y dye was used to analyze depth of the penetration after the treatment. Depth of penetration was displayed by microscopic observation and FE-SEM/EDS observation after skin sectioning. Absorption of eosin Y inside the skin measured by FE-SEM/EDS and optical microscope was consistent with absorption of adenosine measured by using Franz diffusion cell and HPLC. Iontophoretic pre-treatment followed by plasma treatment caused increased drug absorption in stratum corneum and plasma treatment itself increased drug penetration through the epidermal layer of the skin.

键词: microplasma, FeSO₄, DMSO, iontophoresis, SEM, EDS, frozen section, Franz cell

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