每年出版 4 期
ISSN 打印: 2155-014X
ISSN 在线: 2155-0158
Dependence of the Endocrine Function of Ischemic Ovarian Tissue Transplant on the Lipid Peroxidation Intensity
摘要
Correlation between endocrine function, morphology, and volume changes in the structure of ovarian tissue, as well as lipid peroxidation intensity at different temperature and time parameters in ischemia modeling were investigated. It has been revealed that the morphological transformations after 2 hours of ischemia at 37°C and 24 hours at 4°C are reversible. In this case, a significant increase in the concentration of sex hormones in animals-recipients is observed relative to castrated ones after heterotopic transplantation. An increase in the duration of ischemia up to 4 hours at 37°C and to 48 hours at 4°C leads to irreversible morphological transformation and failure of endocrine function, except for the tissue incubation (4°C, 48 hours) in a mannitol-containing solution (MCS), where the accumulation level of thiobarbituric acid reactive substances (TBARS) is 2.5–3 times lower. This data correlates with a significant growth of estradiol and progesterone concentrations in recipient animals. Thus, the combined protective effect of hypothermia and mannitol in ischemia has been demonstrated.