每年出版 4 期
ISSN 打印: 2155-014X
ISSN 在线: 2155-0158
Effect of Agmatine on Actin Polymerization Dynamics in WGA-Stimulated Leukocytes under Diabetes Mellitus in Rats
摘要
We examined the effect of agmatine on the aggregation ability of leukocytes induced by sialospecific lectin of wheat germ agglutinin (WGA) and the process of polymerization of actin cytoskeleton of leukocyte in normal conditions and in experimental diabetes mellitus (DM) after 0.5, 1 and 3 min of preincubation of these cells with the WGA. The experiments have revealed that agmatine administration to animals with DM leads to a 2.7- and 3.2-fold increase in the aggregate size and aggregation velocity of leukocytes, respectively, compared to diabetic rats not receiving this polyamine injections. The results obtained testify to the increase in the content of sialoglycoconjugates and N-acetyl-D-glucosamine containing glycans on leukocyte surface. It should be noted that leukocytes, activated by WGA stimulatory signals, contain more polymerized actin compared to the cells from control animals treated with this polyamine and leukocytes of animals with DM. Our data suggest that under conditions of DM the agmatine insertion makes the recovery of affinity binding of complementary ligands with sialoglycoconjugates with subsequent intracellular transduction of lectin-induced signal which causes the reorganization of the actin cytoskeleton in leukocytes.
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Sabadashka Mariya, Nagalievska Mariia, Sybirna Nataliia, Tyrosine nitration as a key event of signal transduction that regulates functional state of the cell, Cell Biology International, 45, 3, 2021. Crossref