每年出版 4 期
ISSN 打印: 2155-014X
ISSN 在线: 2155-0158
Expression of Proteasome Subunits PSMB5 and PSMB9 mRNA in Hippocampal Neurons: Link with Apoptosis and Necrosis
摘要
There is a huge body of evidence showing that long-termed diabetes mellitus is followed by hippocampal dysfunction. The goal of this work was to investigate the expression of proteasome subunits PSMB5 and PSMB9 mRNA in CA1, CA2 and CA3 areas of hippo-campus in parallel with processes of cell death (apoptosis and necrosis) in development dynamics of streptozotocine-induced diabetes. We have studied hippocampal neurons using chromatine dye Hoechst-33342 and immunohistochemical detection of apoptotic cell death marker caspase-3. At day 3 and 7 after injection of streptosotocine we have performed visualization of caspase-3- immunopositive neurons showing signs of neurodegeneration in hippocampal sections using confocal microscope Olympus FV1000. The rate of proteasome subunits PSMB5 and PSMB9 mRNA expression was determined with RT-PCR. Results indicated elevation of PSMB9 mRNA content (from 4.8 ± 0.4 arbU up to 20.0 ± 4.9 arbU on day 3 and up to 20.3 ± 5.1 arbU on day 7). Maximal number of cells with signs of chromatin condensation was observed at day 3 and day 7 in CA2 and CA3 area (11.5% and 12.5% respectively). That indicates an intensification of proapoptotic processes. Summarizing above results we can reach the conclusion that during the first week of Diabetes mellitus development hippocampal cells undergo the process of impairment and degeneration.